mProX™ Human GRIN1/GRIN2B Stable Cell Line

Product Information

Target Protein

GRIN1/GRIN2B

Target Family

Glutamate Receptor

Target Protein Species

Human

Host Cell Type

COS-7; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Neurodevelopmental Disorder

Gene ID

2902

UniProt ID

Q05586

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The NMDA receptor subunit NR2B is encoded by GRIN2B. De novo mutations in this gene have been linked to Lennox-Gastaut syndrome, infantile spasms, West syndrome, and intellectual disability with focal epilepsy. It's interesting to note that GRIN2B mutations appear to have very distinct functional effects than GRIN2A mutations. Three glutamatergic signaling genes have been shown to have genetic variations or different expression patterns, which are linked to the severity of AWS in humans. Alcoholics who have a functional Ser310Ala polymorphism in the GRIK3 gene have delirium tremens. GRIN1 genotypes containing A and the 2108A allele were linked to a history of withdrawal-induced seizures. The GRIN2B gene, which codes for the NR2B subunit of the NMDA receptor, has polymorphisms that have not been linked to alcoholism, seizures brought on by alcohol withdrawal, or delirium tremens. The customized GRIN1/GRIN2B stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Patricia (Verified Customer)

Can the GRIN1/GRIN2B cell line be used in PPI studies? Jul 11 2020

chat Sherry Smith (Creative Biolabs Scientific Support)

Yes, cell lines expressing specific proteins can be used in PPI studies. Jul 11 2020

chat James (Verified Customer)

Does the GRIN1/GRIN2B stable cell line overexpress the full-length GRIN1/GRIN2B? May 19 2021

chat Sherry Smith (Creative Biolabs Scientific Support)

Yes, the GRIN1/GRIN2B stable cell line can be customized to overexpress the full-length GRIN1/GRIN2B. May 19 2021

Published Data

Fig.1 Effects of biallelic GRIN1-DNVs on NMDAR surface expression.

NMDAR surface expression in COS-7 cell line co-transfected with GFP-Grin2b and HA-Grin1 (wildtype and/or mutant) constructs was examined using immunofluorescence.

Ref: Santos-Gómez, Ana, et al. "Paradigmatic De novo GRIN1 variants recapitulate pathophysiological mechanisms underlying GRIN1-related disorder clinical Spectrum." International Journal of Molecular Sciences 22.23 (2021): 12656.

Pubmed: 34884460

DOI: 10.3390/ijms222312656

Research Highlights

They discovered evidence that GRIN2B encephalopathy is frequently related with movement disorder, cortical visual impairment, and MCD, indicating unexpected phenotypic consequences of channelopathies, in addition to previously known hallmarks of intellectual disability, epilepsy, and autism.
Platzer, Konrad, et al. "GRIN2B encephalopathy: novel findings on phenotype, variant clustering, functional consequences and treatment aspects." Journal of medical genetics 54.7 (2017): 460-470.
Pubmed: 28377535 DOI: 10.1136/jmedgenet-2016-104509

A complex mental illness with a high heritability is schizophrenia. The pathophysiology of schizophrenia may involve N-methyl-D-aspartate (NMDA)-type glutamate receptor dysfunction.
Poltavskaya, Evgeniya G., et al. "Study of early onset schizophrenia: Associations of GRIN2A and GRIN2B polymorphisms." Life 11.10 (2021): 997.
Pubmed: 34685369 DOI: 10.3390/life11100997