mProX™ Human GRIN1/GRIN2A Stable Cell Line

Product Information

Target Protein

GRIN1/GRIN2A

Target Family

Glutamate Receptor

Target Protein Species

Human

Host Cell Type

COS-M6; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Neurodevelopmental Disorder

Gene ID

2902

UniProt ID

Q05586

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human GRIN2A gene encodes the protein glutamate [NMDA] receptor subunit epsilon-1. The conventional GluN2A subunit isoform has a considerable length of 1464 amino acids. Alternative splicing can generate the monkey-specific GluN2A-short isoforms, which have 1281 amino acids. Ionotropic glutamate receptors include the N-methyl-D-aspartate (NMDA) receptor class. Long-term potentiation, an activity-dependent improvement in synaptic transmission efficiency believed to underpin some forms of memory and learning, has been linked to the NMDA channel. The main receptor subunit NMDAR1 (GRIN1) and one or more of the four NMDAR2 subunits-NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D)-compose heteromers that make up NMDA receptor channels. The customized GRIN1/GRIN2A stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Sharon (Verified Customer)

What host cells are typically used for the GRIN1/GRIN2A stable cell line? Aug 17 2020

chat Sherry Smith (Creative Biolabs Scientific Support)

Mammalian cells such as HEK293 or CHO-K1. Aug 17 2020

chat Amy (Verified Customer)

What is the BSL of the GRIN1/GRIN2A cell line? Dec 13 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

The BSL of the GRIN1/GRIN2A cell line is Level 1. Dec 13 2022

Published Data

Fig.1 Subcellular distribution of GRIN1 and Goα.

Following SDS-PAGE resolution, subcellular fractions were immunoblotted using the designated antibodies. COS cells that were transfected to express Goα and GRIN1.There are five lanes: one for untransfected COS cells (1000 × g supernatant); another for transfected COS cells (1000 × g supernatant); a fourth for transfected cells (10,000 × g supernatant); and a fifth for transfected cells (100,000 × g pellet).

Ref: Chen, Linda T., Alfred G. Gilman, and Tohru Kozasa. "A candidate target for G protein action in brain." Journal of Biological Chemistry 274.38 (1999): 26931-26938.

Pubmed: 10480904

DOI: 10.1074/jbc.274.38.26931

Research Highlights

Changes in the N-methyl-d-aspartate receptor (NMDAR) component GluN2A, which is encoded by GRIN2A, have been linked to a variety of neurodevelopmental diseases, including epilepsy and illnesses with strong speech-related symptoms.
Strehlow, Vincent, et al. "GRIN2A-related disorders: genotype and functional consequence predict phenotype." Brain 142.1 (2019): 80-92.
Pubmed: 30544257 DOI: 10.1093/brain/awy304

Research employing a diverse range of techniques, including molecular cytogenetics, next-generation sequencing technologies, and functional assays, has enabled the detection and confirmation of genetic mutations in the GRIN2A gene that may be directly responsible for a number of EAS illnesses.
Lesca, Gaetan, et al. "Update on the genetics of the epilepsy-aphasia spectrum and role of GRIN2A mutations." Epileptic Disorders 21 (2019): S41-S47.
Pubmed: 31149903 DOI: 10.1684/epd.2019.1056