mProX™ Human GPR68 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 ER stress is induced by acidosis activated OGR1-mediated signalling.
After 4-6 hours of incubation in pH 7.6 serum free medium, Caco-2 cells were exposed to varied pH medium. Tunicamycin was applied to vector control Caco-2 (VC) and OGR1 overexpressing Caco-2 cells for 24 hours at the given doses. Western blotting was done after total protein was extracted.
Ref: Maeyashiki, Chiaki, et al. "Activation of pH-Sensing Receptor OGR1 (GPR68) Induces ER Stress Via the IRE1 α/JNK Pathway in an Intestinal Epithelial Cell Model." Scientific reports 10.1 (2020): 1438.
Pubmed: 31996710
DOI: 10.1038/s41598-020-57657-9
Research Highlights
A number of cellular processes are controlled by GPR68, a proton-sensing G-protein-coupled receptor (GPCR) that reacts to extracellular acidity. One distinctive feature of the tumor microenvironment (TME) is acidosis. The expression of GPR68 is markedly increased in many different cancers.
Wiley, Shu Z., et al. "GPR68: an emerging drug target in cancer." International journal of molecular sciences 20.3 (2019): 559.
Pubmed:
30696114
DOI:
10.3390/ijms20030559
One of these is the proton receptor GPR68, which is lacking the small chemical modulators needed to examine its biology. Here, we identified the benzodiazepine medication lorazepam as a non-selective GPR68 positive allosteric modulator using yeast-based screens against GPR68.
Huang, Xi-Ping, et al. "Allosteric ligands for the pharmacologically dark receptors GPR68 and GPR65." Nature 527.7579 (2015): 477-483.
Pubmed:
26550826
DOI:
10.1038/nature15699