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  • mProX™ Human GPR68 Stable Cell Line

    [CAT#: S01YF-0923-KX48]
    Product Category:
    Membrane Protein Stable Cell Lines
    Subcategory:
    GPCR Cell Lines

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    Based on this stable cell line, we also provide cell-based in vitro assays to evaluate the effects of your compounds or antibodies.

    Sub Cat Product Name Target Protein Species Host Cell Type Assay Types Inquiry Datasheet
    S01YF-1122-KX808 Magic™ Mouse GPR68 in Vitro cAMP Assay Mouse 1321N1 cAMP Assay
    S01YF-1122-KX809 Magic™ Rat GPR68 in Vitro cAMP Assay Rat 1321N1 cAMP Assay

    Product Information

    Target Protein
    GPR68
    Target Family
    SPC/LPC/Proton-sensor Family
    Target Protein Species
    Human
    Host Cell Type
    Caco-2; CHO-K1; HEK293
    Target Classification
    GPCR Cell Lines
    Target Research Area
    CNS Research; Orphan Receptor Research
    Related Diseases
    Amelogenesis Imperfecta
    Gene ID
    UniProt ID

    Product Properties

    Biosafety Level
    Level 1
    Activity
    Yes
    Quantity
    10⁶ cells per vial
    Applications
    GPR68 was identified as a proton-sensing GPCR that affects the function of many different cell types in response to extracellular acidity. GPR68 sensing of H+ is assumed to occur through protonation of histidine residues, which results in conformational changes and receptor activation, in contrast to the ligand-receptor binding pocket interaction of other GPCRs. The mRNA expression of GPR68 is elevated in many cancers, and it is expressed in a variety of organs. New evidence suggests that GPR68 plays important functions in tumor biology. GPR68 was first discovered to be a metastasis suppressor gene in prostate cancer, but it has also been shown to support carcinogenesis in melanoma and prostate cancer. Additionally, GPR68 controls the growth and proliferation of medulloblastoma cells and may be a mediator of lenalidomide response in MDS. The customized GPR68 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

    Protocols

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    Customer Reviews

    chat Eartha

    I customized a GPR68 cell line and it's very good. Aug 16 2023

    chat Verified Customer

    chat Vanessa

    This cell line is very suitable for studying the GPR68 signaling pathway. Jul 11 2023

    chat Verified Customer

    FAQ

    Any questions about our products? Please visit our frequently asked questions page.

    Published Data

    Fig.1 ER stress is induced by acidosis activated OGR1-mediated signalling.

    After 4-6 hours of incubation in pH 7.6 serum free medium, Caco-2 cells were exposed to varied pH medium. Tunicamycin was applied to vector control Caco-2 (VC) and OGR1 overexpressing Caco-2 cells for 24 hours at the given doses. Western blotting was done after total protein was extracted.

    Ref: Maeyashiki, Chiaki, et al. "Activation of pH-Sensing Receptor OGR1 (GPR68) Induces ER Stress Via the IRE1 α/JNK Pathway in an Intestinal Epithelial Cell Model." Scientific reports 10.1 (2020): 1438.

    Pubmed: 31996710

    DOI: 10.1038/s41598-020-57657-9

    Research Highlights

    A number of cellular processes are controlled by GPR68, a proton-sensing G-protein-coupled receptor (GPCR) that reacts to extracellular acidity. One distinctive feature of the tumor microenvironment (TME) is acidosis. The expression of GPR68 is markedly increased in many different cancers.
    Wiley, Shu Z., et al. "GPR68: an emerging drug target in cancer." International journal of molecular sciences 20.3 (2019): 559.
    Pubmed: 30696114   DOI: 10.3390/ijms20030559

    One of these is the proton receptor GPR68, which is lacking the small chemical modulators needed to examine its biology. Here, we identified the benzodiazepine medication lorazepam as a non-selective GPR68 positive allosteric modulator using yeast-based screens against GPR68.
    Huang, Xi-Ping, et al. "Allosteric ligands for the pharmacologically dark receptors GPR68 and GPR65." Nature 527.7579 (2015): 477-483.
    Pubmed: 26550826   DOI: 10.1038/nature15699

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR CLINICAL PROCEDURES" For licensing inquiries, please contact
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