mProX™ Human GNRHR Stable Cell Line

Product Information

Target Protein

GNRHR

Target Family

Gonadotrophin-releasing Hormone Family

Target Protein Species

Monkey

Host Cell Type

COS-7;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Metabolic Research

Related Diseases

Hypogonadotropic Hypogonadism 7 With Or Without Anosmia;Infertility

Gene ID

Monkey: 711577

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Gonadotropin-releasing hormone receptor (GNRHR) is pivotal in the regulation of the reproductive system. Polymorphisms in the GNRHR gene have been associated with fertility traits in livestock, suggesting its potential in marker-assisted selection. Furthermore, GNRHR has been linked to conditions like congenital idiopathic hypogonadotropic hypogonadism, emphasizing its role in reproductive health. Additionally, the association between GNRHR gene variants and conditions like polycystic ovary syndrome has been explored, highlighting its significance in reproductive disorders.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Paul (Verified Customer)

What is the significance of the GnRHR expression in the human spinal cord? Feb 22 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

The presence of gonadotropin-releasing hormone receptor (GnRHR) in the spinal cord suggests a potential extrapituitary functional role that is independent of the reproductive system. Feb 22 2020

chat Paul (Verified Customer)

How do GNRHR gene variants impact congenital idiopathic hypogonadotropic hypogonadism (CHH)? Jul 26 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

Biallelic variants in the GNRHR gene can lead to a severe reproductive phenotype characterized by low gonadotropin levels without nonreproductive features or oligogenicity. However, the role of monoallelic GNRHR variants in CHH remains uncertain for some reported variants. Jul 26 2021

Published Data

Fig.1 An in-depth exploration of the p.Thr269Met GNRHR mutation's impact on hypogonadotropic hypogonadism, unraveling its functional intricacies.

COS-7 cells underwent transfection with either the wild-type (WT) or the p.Thr269Met mutant GnRHR, followed by GnRH stimulation for one hour, featuring incremental concentrations (ranging from 10^-10 to 10^-7 M) of a synthetic GnRH ligand [log M]. Intracellular inositol phosphate (IP) accumulation was quantified in triplicates of lysed cells utilizing the IP3 Elisa kit at 450 nm. A notably diminished dose-response induction of IP accumulation was observed in comparison to WT GNRHR-transfected cells, consistently registering lower levels than those elicited by WT-GNRHR transfection. These findings were graphed on GraphPad Prism relative to the maximum response of a standard IP curve, revealing statistical significance (*, p < 0.05) denoting reduced IP accumulation in the p.Thr269Met mutant compared to the WT GnRHR.

Ref: Correa-Silva, Silvia Regina, et al. "A novel GNRHR gene mutation causing congenital hypogonadotrophic hypogonadism in a Brazilian kindred." Journal of Neuroendocrinology 30.12 (2018): e12658.

Pubmed: 30415482

DOI: 10.1111/jne.12658

Research Highlights

Santos LC, et al. "Kisspeptin treatment reverses high prolactin levels and improves gonadal function ." Scientific reports, 2023.
The effects of kisspeptin-10 (Kp10) administration on restoring gonadal function in hypothyroid male rats were evaluated in this study. Hypothyroidism was induced using 6-propyl-2-thiouracil (PTU) for three months, after which half of the hypothyroid rats were treated with Kp10 in the last month. Results showed that hypothyroidism decreased testicular and sex gland mass, as well as sperm morphology, motility, and vigor. It also reduced Kiss1 and Kiss1r protein and gene expression and affected various hormone levels in the pituitary and hypothalamus. Treatment with Kp10 showed improvements in testicular and seminal vesicle morphology, sperm quality, hormone levels, and gene expression in the testis, pituitary, and hypothalamus in hypothyroid rats. These findings suggest that Kp10 treatment can reverse the inhibitory effects of hypothyroidism on the male gonadal axis and improve sperm quality.
Pubmed: 37798396 DOI: 10.1038/s41598-023-44056-z

Olea GB, et al. "Early gonadogenesis in Columba livia (Birds: Columbiformes): migration, ." Development, growth & differentiation, 2023.
In avian species, the process of migration and colonization of primordial germ cells (PGCs) in the gonadal crest is carried out through the bloodstream. Previous studies have used DDX4, SSEA1, and Oct4 to identify PGCs, while other molecules such as N-cadherin, GnRHR, and 3betaHSD have been used during gonadal development in mice and birds. However, their role in early gonadogenesis in birds is still poorly understood. This study aims to analyze the differential immunodetection of N-cadherin, Oct4, GnRHR, and 3betaHSD in Columba livia embryos during the migration and colonization of PGCs in the genital crest and early gonadogenesis stages. Immunohistochemistry was performed in corresponding histological sections of C. livia from stages (S) 15 to S40. The results showed that N-cadherin, Oct4, GnRHR, and 3betaHSD were immunodetected in the germ line of C. livia and were useful markers for identifying PGCs during their migration and colonization in the genital crest, as well as in early gonadogenesis. This study presents an effective model for understanding early gonadogenesis in altricial species and highlights the potential of these markers for future research.
Pubmed: 37795634 DOI: 10.1111/dgd.12895