mProX™ Human GHSR Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Patrick Liam (Creative Biolabs Scientific Support)
James (Verified Customer)
Patrick Liam (Creative Biolabs Scientific Support)
Published Data
Fig.1 Adenoviral GHSR-1a promotes angiogenesis and tube formation.
Tube formation by human umbilical endothelial cells (HUVECs) was evaluated in regular culture conditions and under ischemic stress post-adenoviral transfection of either GHSR-1a or a control plasmid for a 24-hour period. Scale bars measure 50 µm. Notably, *P<0.05 was observed compared to Ad-null during hypoxic conditions.
Ref: Yuan, Ming-Jie, et al. "GHSR-1a is a novel pro-angiogenic and anti-remodeling target in rats after myocardial infarction." European journal of pharmacology 788 (2016): 218-225.
Pubmed: 27343377
DOI: 10.1016/j.ejphar.2016.06.032
Research Highlights
Beheshti S, et al. "Differential impact of a ghrelin receptor antagonist or inverse agonist in the ." Epilepsy research, 2023.
The peptide ghrelin has been found to have an effect on seizures, but there is no consensus on its exact influence in controlling seizure severity. A study was conducted to assess the effect of a ghrelin receptor antagonist and inverse agonist on seizures induced by electrical kindling. In adult male rats, electrodes were implanted in the skull or the basolateral amygdala, and a rapid kindling protocol was used. The rats were treated with either D-Lys-3-GHRP-6 or [D-Arg, D-phe, D-Trp, heu] substance P (D-SP) as the antagonist or inverse agonist, respectively. The results showed that antagonism of the ghrelin receptor in the amygdala increased seizure induction in a dose-dependent manner and led to spontaneous seizures. On the other hand, D-SP had a dose-dependent anticonvulsant effect, as evidenced by a decrease in seizure duration. These findings suggest that different GHSR ligands may have varying effects on seizure control, highlighting the importance of considering the specific ghrelin system in modulation of seizures.
Pubmed:
37793283
DOI:
10.1016/j.eplepsyres.2023.107234
Ringuet MT, et al. "Sites and mechanisms of action of colokinetics at dopamine, ghrelin and serotonin ." The Journal of physiology, 2023.
In this study, the effects of dopamine, 5-hydroxytryptamine (5-HT), and ghrelin receptor agonists on colorectal motility were investigated. It was hypothesized that these agonists act on parasympathetic preganglionic neurons (PGNs) in the lumbosacral spinal cord. The researchers aimed to identify which specific neurons in this region express these receptors, their neuronal inputs, and if these agonists stimulate them. The results revealed that dopamine, serotonin, and ghrelin receptor transcripts are present in the same PGNs, and that these neurons have closely associated tyrosine hydroxylase and serotonin boutons. Whole cell electrophysiology experiments showed that these agonists induce an inward excitatory current in overlapping populations of lumbosacral PGNs. Furthermore, the excitatory effects of dopamine were reversed by GHSR antagonism, indicating that ghrelin receptors modulate dopamine effects at D2 receptors. These findings suggest that lumbosacral PGNs are the site at which actions of these endogenous ligands converge, and that ghrelin receptors play a regulatory role in dopaminergic signaling.
Pubmed:
37772438
DOI:
10.1113/JP285217