mProX™ Human GABRA4 Stable Cell Line

Product Information

Target Protein

GABRA4

Target Family

GABAA

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Status Epilepticus; Autism

Gene ID

2557

UniProt ID

P48169

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The GABRA4 gene in humans codes for the protein known as gamma-aminobutyric acid receptor subunit alpha-4. In a study, mice with the wild-type and knockout GABRA4 genes were compared. It was discovered that traits linked to autism spectrum disorder (ASD) were seen when the GABRA4 gene was removed. Compared to wild-type mice, they include reduced social engagement and enhanced spatial cognition. An examination of the hippocampus transcriptome in knockout mice revealed elevated N-methyl-D-aspartate receptor activation. This contributes to consciousness and the learning that leads to those traits. The customized GABRA4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Rebecca (Verified Customer)

How is GABRA4 associated with Alzheimer's Disease and other neurological disorders? Mar 21 2020

chat Sherry Smith (Creative Biolabs Scientific Support)

A severe reduction of GABRA4 has been observed in a subset of patients with Alzheimer's Disease (AD) and Parkinson's Disease (PD). Mar 21 2020

chat Nancy (Verified Customer)

What is the role of GABRA4 in melanoma suppression? Aug 22 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

GABRA4 is inhibited by endogenous DOPA, which regulates melanocyte proliferative capacity and susceptibility to malignant transformation, indicating a potential role in melanoma suppression. Aug 22 2023

Published Data

Fig.1 GABRA4 is essential to control pathologic inflammation during mycobacterial infection.

The lung tissue of Mtb-infected gabra4 CKO mice had significantly greater levels of proinflammatory cytokine and chemokine mRNA expressions (i.e., interleukin (Il)6, CC motif chemokine ligand (Ccl)2, and C-X-C motif ligand (Cxcl)5) than did the lung tissue of gabra4 WT mice.

Ref: Kim, Jin Kyung, et al. "Gamma-aminobutyric acid type A receptor alpha 4 coordinates autophagy, inflammation, and immunometabolism to promote innate immune activation." Autophagy Reports 2.1 (2023): 2181915.

Research Highlights

A neurological developmental disease, autism spectrum disorder (ASD) is characterized by poor social interaction and communication, atypical IQ, and co-occurring seizures.
Fan, Cuixia, et al. "Transcriptomics of Gabra4 knockout mice reveals common NMDAR pathways underlying autism, memory, and epilepsy." Molecular autism 11.1 (2020): 1-17.
Pubmed: 32033586 DOI: 10.1186/s13229-020-0318-9

Different types of epilepsy and neurodevelopmental abnormalities are caused by variations in the genes encoding the γ-aminobutyric acid A (GABAA) receptor. Currently, there is no known connection between GABRA4, which codes for the α4-subunit, and any monogenic disorder.
Vogel, Florian D., et al. "A de novo missense variant in GABRA4 alters receptor function in an epileptic and neurodevelopmental phenotype." Epilepsia 63.4 (2022): e35-e41.
Pubmed: 35152403 DOI: 10.1111/epi.17188