mProX™ Human FSHR Stable Cell Line

Santos LC, et al. "Kisspeptin treatment reverses high prolactin levels and improves gonadal function ." Scientific reports, 2023.
The effectiveness of kisspeptin-10 (Kp10) in restoring gonadal function in hypothyroid male rats was evaluated in this study. Hypothyroidism was induced in male rats using 6-propyl-2-thiouracil (PTU) for a period of three months. In the last month, half of the hypothyroid animals were treated with Kp10. The results showed that hypothyroidism caused a decrease in testicular and sex gland mass, inhibited the proliferation of the seminiferous epithelium, and impacted sperm morphology, motility, and vigor. Furthermore, there was a decrease in plasma LH and testosterone levels, and an increase in prolactin secretion in the hypothyroid rats. This condition also resulted in reduced Kiss1 and Kiss1r protein and gene expression, as well as Star and Cyp11a1 mRNA levels in the testis. Additionally, it decreased Lhb, Prl, and Drd2 expression, while increasing Tshb and Gnrhr expression in the pituitary gland. In the hypothalamus, hypothyroidism increased Pdyn and Kiss1r expression, while reducing Gnrh1 expression. Treatment with Kp10 in hypothyroid rats showed improvements in testicular and seminal vesicle morphology, sperm quality, and levels of prolactin, LH, and testosterone. Furthermore, Kp10 treatment increased expression of Kiss1, Kiss1r, Fshr, and Nr5a1 in the testis, as well as pituitary Kiss1 expression. These findings demonstrate the inhibitory effects of hypothyroidism on the male gonadal axis and sperm quality, and suggest that treatment with Kp10 can reverse these effects and improve overall gonadal function in hypothyroid rats.
Pubmed: 37798396   DOI: 10.1038/s41598-023-44056-z

Yoshita S, et al. "Unkeito promotes follicle development by restoring reduced follicle-stimulating ." Frontiers in endocrinology, 2023.
Polycystic ovary syndrome (PCOS) is a common condition characterized by irregular menstruation and infertility caused by improper development and ovulation of follicles. It is thought that PCOS is triggered by decreased expression of the follicle-stimulating hormone receptor (FSHR) in granulosa cells (GCs), although the exact underlying mechanism is still unknown. Unkeito (UKT), a traditional Japanese medicine, has been used to treat irregular menstruation in PCOS patients. In this study, the researchers aimed to confirm the effectiveness of UKT in treating PCOS by investigating its impact on FSH responsiveness. To do so, a rat model of PCOS was generated and then treated with daily UKT or a normal diet starting at 3 weeks of age. The researchers evaluated the estrous cycle, hormone levels, and ovarian structure of the rats to compare the effects of UKT on the PCOS phenotype. Further investigation was done using molecular, genetic, and immunohistological analyses on ovarian tissue and primary cultured GCs from both normal and PCOS model rats. The results showed that UKT increased the number of antral and preovulatory follicles and restored the irregular estrous cycle in PCOS rats. Additionally, UKT increased the expression of FSHR and bone morphogenetic proteins (BMPs) -2 and -6 in ovarian GCs of PCOS rats. This suggests that UKT may enhance FSH responsiveness and potentially alleviate the PCOS phenotype by regulating FSHR function in the small antral follicles.
Pubmed: 37790609   DOI: 10.3389/fendo.2023.1228088