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  • mProX™ Human FFAR3 Stable Cell Line

    [CAT#: S01YF-0923-PY68]
    Product Category:
    Membrane Protein Stable Cell Lines
    Subcategory:
    GPCR Cell Lines

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    Product Information

    Target Protein
    FFAR3
    Target Family
    Free Fatty Acid Family
    Target Protein Species
    Human
    Host Cell Type
    NCM460;CHO-K1;HEK293
    Target Classification
    GPCR Cell Lines
    Target Research Area
    Digestive and Renal Research
    Related Diseases
    Diversion Colitis;Anaerobic Meningitis
    Gene ID
    Human: 2865

    Product Properties

    Biosafety Level
    Level 1
    Activity
    Yes
    Quantity
    10⁶ cells per vial
    Applications
    FFAR3, also known as GPR41, is intricately linked with various metabolic and immune processes. One of its notable roles is in the heart, where it has been associated with dampening of FFAR3 signaling in cardiomyocytes, influencing cAMP levels and pro-inflammatory interleukin production. Additionally, the gut-lung axis has been highlighted, where gut-derived short-chain fatty acids (SCFAs) can influence lung immune tone, with FFAR3 playing a pivotal role in this interaction. Furthermore, FFAR3's expression in vagal neurons has been linked to the regulation of feeding behavior, suggesting its potential role in appetite control.

    Protocols

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    FAQ

    chat Anthony (Verified Customer)

    How does FFAR3 contribute to the regulation of pancreatic endocrine secretion in mice? Aug 11 2021

    chat Patrick Liam (Creative Biolabs Scientific Support)

    The effects of certain compounds on somatostatin secretion suggest that it may be possible to manipulate pancreatic secretion pharmacologically with agonists of the FFAR2 and 3 receptors. Aug 11 2021

    chat Paul (Verified Customer)

    What is the significance of FFAR3 in cardiac regulation? Nov 13 2021

    chat Patrick Liam (Creative Biolabs Scientific Support)

    Pressure overload upregulates cardiac RGS4 to protect the heart against FFAR3-mediated inflammation and elevated sympathetic nervous system activity. Nov 13 2021

    Published Data

    Fig.1 The mitigation of reactive oxygen species (ROS) damage in colonic epithelial cells by propionate occurred irrespective of any involvement of G protein-coupled receptors.

    Assessment of ROS formation in NCM460 cells was conducted via DCFH-DA staining, involving both wild-type and NCM460 cells with knockdown of FFAR3. These cells were subjected to treatment with TNF-α and propionate.

    Ref: Chen, Liuying, et al. "The role of NADPH oxidase 1 in alcohol-induced oxidative stress injury of intestinal epithelial cells." Cell Biology and Toxicology (2022): 1-20.

    Pubmed: 35639301

    DOI: 10.1007/s10565-022-09725-1

    Research Highlights

    Gudneppanavar R, et al. "Epigenetic histone modification by butyrate downregulates KIT and attenuates mast ." Journal of cellular and molecular medicine, 2023.
    Butyrate, a short-chain fatty acid produced from bacterial fermentation, has been shown to reduce lung inflammation in asthma models by suppressing the activation of mast cells (MCs). MCs are known to play a crucial role in the inflammatory response to allergens in asthma. Their survival and proliferation are regulated by the growth factor, stem cell factor (SCF), through the KIT receptor. Although previous studies have demonstrated the ability of butyrate to suppress MC activation, its mechanism of action on SCF signalling and MC function is not yet fully understood. In this study, the researchers found that butyrate treatment caused modifications in MC histones through butyrylation and acetylation, as well as inhibited histone deacetylase (HDAC) activity. Additionally, butyrate treatment resulted in the downregulation of KIT receptor and its associated phosphorylation, thus attenuating SCF-mediated MC proliferation and cytokine secretion. Further investigation revealed that butyrate's suppression of MC function is mediated by its inhibition of the KIT/p38/Erk signalling pathway and its epigenetic effects on histones, acting as an HDAC inhibitor rather than through its traditional butyrate receptors, GPR41 and GPR43. The researchers suggest that these findings support butyrate as a potential treatment for allergy and asthma through its epigenetic modulation of MC function.
    Pubmed: 37603611   DOI: 10.1111/jcmm.17924

    Gao K, et al. "Oral administration of Bifidobacterium longum WHH2270 ameliorates type 2 diabetes ." Journal of food science, 2023.
    A strain of Bifidobacterium longum known as WHH2270 has been identified as having strong potential as a hypoglycemic agent for treating type 2 diabetes mellitus. Its high level of inhibitory activity against alpha-glucosidase makes it a promising candidate for managing blood glucose levels. This abstract presents a promising avenue of research for the use of Bifidobacterium longum WHH2270 as a potential treatment for T2DM.
    Pubmed: 37548634   DOI: 10.1111/1750-3841.16727

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR CLINICAL PROCEDURES" For licensing inquiries, please contact
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