mProX™ Human DRD5 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 DRD5 knockdown promote autophagic flux.
In HeLa cells with stable DRD5-GFP-3FLAG expression, autophagic flux was assessed by combining DRD5 RNAi with autophagy inhibitors (CQ, 40 μM) over a 2-hour period. Nonspecific bands are denoted by asterisks (*). The experiment was replicated a minimum of three times, and representative Western blots were presented. Densitometric analysis was conducted, and quantification results are indicated beneath the respective blots or in separate panels, with significance levels denoted as * p < 0.05 and ** p < 0.01.
Ref: Wang, Dongmei, et al. "Dopamine receptor subtypes differentially regulate autophagy." International journal of molecular sciences 19.5 (2018): 1540.
Pubmed: 29786666
DOI: 10.3390/ijms19051540
Research Highlights
Ye W, et al. "Transcriptome analysis revealed the existence of family-specific regulation of ." Genomics, 2023.
In this study, the authors investigated the role of ahsg2 and other genes in regulating the growth of grass carp. By analyzing genetic data from zebrafish and conducting further research on grass carp families, the researchers found that the effects of ahsg2 on growth vary among individuals. They also identified specific genetic pathways that may contribute to the growth differences observed in the FF and SS families. This novel study sheds light on the genetic mechanisms underlying grass carp growth and provides valuable insight into nutrient metabolism and brain development.
Pubmed:
37714387
DOI:
10.1016/j.ygeno.2023.110706
Polzin BJ, et al. "Distinct patterns of gene expression in the medial preoptic area are related to ." BMC neuroscience, 2023.
The study focused on gregarious song, a social behavior performed by adult European starlings (Sturnus vulgaris) and facilitated by a low-stress, positive affective state. The role of the medial preoptic area (mPOA) in the production of gregarious song was investigated, specifically examining the potential involvement of neurotransmitter, neuromodulator, and hormonal pathways. RNA sequencing was used to identify differentially expressed genes in the mPOA of male and female starlings who sang at high rates. Results showed an association between dopaminergic, cholinergic, and GABAergic systems and gregarious song, as well as co-expressing gene clusters related to glutamate signaling. Of note, two genes (GRIN1 and SHANK2) were positively correlated with the performance of gregarious song. This research provides valuable insights into the neurochemical mechanisms involved in gregarious social interactions in starlings and may have implications for understanding similar behaviors in other vertebrate species. Data from this study can also be used by other researchers to compare and identify conserved systems regulating social behavior across species.
Pubmed:
37537543
DOI:
10.1186/s12868-023-00813-4
