mProX™ Human CYSLTR1 Stable Cell Line

Sayour NV, et al. "Droplet Digital PCR Is a Novel Screening Method Identifying Potential Cardiac ." International journal of molecular sciences, 2023.
The need for improved outcomes in heart failure (HF) has prompted the search for novel drug targets. G-protein-coupled receptors (GPCRs) are the largest family of targets for approved drugs, which allows for potential repurposing. This study aimed to investigate differential expressions of 288 cardiac GPCRs using droplet digital PCR (ddPCR) and bulk RNA sequencing (RNAseq) in a rat model of left ventricular pressure-overload. Male Wistar rats subjected to transverse aortic constriction (TAC, n = 5) were compared to sham operated animals (SHAM, n = 5). Results showed significant systolic dysfunction in TAC rats compared to SHAM rats, as determined by echocardiography. RNAseq identified 69 differentially expressed GPCR mRNAs in TAC rats compared to SHAM rats, while ddPCR identified 27 differentially expressed GPCRs. Of these, 8 were identified by both methods, demonstrating correlation. Further investigation of the Prostaglandin-F2alpha-receptor (Ptgfr) using RNA-Scope revealed its presence on cardiomyocytes and fibroblasts in murine hearts. Antagonizing Ptgfr with AL-8810 showed potential for alleviating angiotensin-II-induced cardiomyocyte hypertrophy in vitro. Thus, this study presents a novel screening method using ddPCR to identify potential GPCR targets in HF, and suggests Ptgfr as a promising target for further investigation in HF treatment.
Pubmed: 37762130   DOI: 10.3390/ijms241813826

Zhao H, et al. "Identification of the shared gene signatures between pulmonary fibrosis and ." Frontiers in immunology, 2023.
The present study aimed to identify common key genes and immune characteristics in Pulmonary Fibrosis (PF) and Pulmonary Hypertension (PH) through bioinformatics techniques. Expression profiles were retrieved from the Gene Expression Database and weighted gene co-expression network analysis was used to identify co-expression modules. The ClueGO software was used to enrich and analyze common genes in PF and PH, and a protein-protein interaction (PPI) network was obtained. Differential genes were screened in a separate cohort and intersected with the PPI network. Further, RT-PCR and immune infiltration analysis were performed on the intersecting genes. Results revealed lymphocyte activation as a common pathophysiological feature of PF and PH. Eight common characteristic genes and upregulation of resting CD4 memory T cells were identified. Using ImmPort database and RT-PCR analysis, IGF1 was identified as a potential therapeutic target for both diseases. Knockdown of IGF1 was found to reduce proliferation and apoptosis resistance in cells induced by hypoxia, platelet-derived growth factor-BB, and transforming growth factor-beta1. This study provides insight into common biomarkers and a potential candidate gene for targeted therapy of PF and PH.
Pubmed: 37731513   DOI: 10.3389/fimmu.2023.1197752