mProX™ Human CSF1R Stable Cell Line

Product Information

Target Protein

CSF1R

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

SKBR3; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research; Reproductive Research

Related Diseases

Leukoencephalopathy, Hereditary Diffuse, With Spheroids 1 and Brain Abnormalities, Neurodegeneration, And Dysosteosclerosis. Among its related pathways are GPCR Pathway and ERK Signaling

Gene ID

1436

UniProt ID

P07333

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human CSF1R gene encodes the cell-surface protein known as colony stimulating factor 1 receptor (CSF1R), sometimes referred to as CD115 and macrophage colony-stimulating factor receptor (M-CSFR). Two ligands, colony stimulating factor 1 (CSF-1) and interleukin-34 (IL-34), can activate the CSF1R receptor. Myeloid cells have high expression levels of CSF1R, and both in vivo and in vitro myeloid cell types cannot survive, proliferate, or differentiate without CSF1R signaling. Treatments for cancer, neurodegeneration, and inflammatory bone diseases all target CSF1R signaling, which is implicated in a variety of diseases. The customized CSF1R stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Barbara

The CSF1R cell line is easy to use and has become an essential tool in my lab. Mar 20 2020

chat Verified Customer

chat Michael

The CSF1R cell line allowed me to perform rigorous experiments with consistent results. Oct 31 2021

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 CSF-1R localizes in the nucleus of SKBR3 breast cancer cells.

Localization of CSF-1R in SKBR3 cells using cell fractionation. As previously mentioned, 50 nM siCONTROL non-targeting pool or 50 nM SMART-pool small interfering RNA (siRNA) targeting CSF-1R mRNA were used to achieve gene silence.

Ref: Barbetti, Valentina, et al. "Chromatin-associated CSF-1R binds to the promoter of proliferation-related genes in breast cancer cells." Oncogene 33.34 (2014): 4359-4364.

Pubmed: 24362524

DOI: 10.1038/onc.2013.542

Research Highlights

This study examines the processes relating CSF1R mutations to pleiotropic effects on postnatal growth and development, as well as the evidence that CSF1R is exclusively expressed in mononuclear phagocytes.
Hume, David A., et al. "Phenotypic impacts of CSF1R deficiencies in humans and model organisms." Journal of leukocyte biology 107.2 (2020): 205-219.
Pubmed: 31330095 DOI: 10.1002/JLB.MR0519-143R

A patient with a frameshift mutation exhibits haploinsufficiency of the colony-stimulating factor-1 receptor (CSF1R), which makes it easier to develop a Csf1r haploinsufficient animal model. The pathologic, radiologic, and clinical characteristics that these animals exhibit are similar to those of CSF1R-mutant human patients.
Konno, Takuya, et al. "CSF1R-related leukoencephalopathy: a major player in primary microgliopathies." Neurology 91.24 (2018): 1092-1104.
Pubmed: 30429277 DOI: 10.1212/WNL.0000000000006642