mProX™ Human CRHR1 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 CRHR1 knockdown inhibited H2O2-increased cerebral endothelial permeability in vitro.
Endothelial permeability in bEnd3 cells infected with lentivirus was assessed through the FITC-dextran transwell assay. After serum starvation of lentivirus-infected bEnd3 cells for 4 hours, they were exposed to H2O2 (2 mM) for 1 hour. Fluorescence intensity was standardized against shNC-infected cells treated with the vehicle. The data, represented as mean ± SEM, were derived from four groups. Significant differences were denoted as **P < 0.01, and ***P < 0.001; while #P < 0.05, ##P < 0.01, and ###P < 0.001 indicated other noteworthy observations.
Ref: Cao, Changchun, et al. "Activation of CRHR1 contributes to cerebral endothelial barrier impairment via cPLA2 phosphorylation in experimental ischemic stroke." Cellular Signalling 66 (2020): 109467.
Pubmed: 31715260
DOI: 10.1016/j.cellsig.2019.109467
Research Highlights
Kuznetsov A, et al. "Genetic Contributors to PTSD: the Role of SNVs, Gene Interactions and Haplotypes ." Psychiatria Danubina, 2023.
Post-traumatic stress disorder (PTSD) is a mental health condition characterized by trauma or stressors and has a significant impact on society. The authors conducted a review to identify genetic markers associated with PTSD, which could inform future prevention and treatment strategies. A search of the PubMed database yielded 547 articles, of which 20 met the inclusion criteria. Results showed significant correlations between PTSD and FKBP5 variants, as well as gene-gene interactions between DRD and OXTR-DRD. However, understanding of the polygenic and epigenetic mechanisms underlying PTSD remains limited and should be a focus of future research.
Pubmed:
37800217
DOI:
No
Mei L, et al. "Identification of candidate genes and chemicals associated with osteoarthritis by ." Arthritis research & therapy, 2023.
Osteoarthritis (OA) is a prevalent degenerative joint disease affecting seniors, causing persistent discomfort and disability. Multiple risk factors contribute, such as aging, obesity, genetic predisposition, and environmental influences. A transcriptome-wide association study (TWAS) and chemical-related gene set enrichment analysis (CGSEA) were conducted to investigate the susceptibility genes and environmental elements. Using summary-level data from knee OA (KOA) and hip OA (HOA) genome-wide association studies, expression weights from the Genotype-Tissue Expression Project (Version 8) were integrated via the FUSION software for both single-tissue and cross-tissue TWAS. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases were utilized to analyze the biological function and pathways of TWAS genes, whilst the human cartilage mRNA expression profiles were used to validate them. In addition, CGSEA analysis was used to identify OA-associated chemicals by combining TWAS results with chemical-related gene sets. Results from both TWAS and CGSEA found 44 and 93 unique susceptibility genes, respectively, for KOA and HOA, across multiple chromosomes, with several showing significantly altered expression in mRNA profiles. These genes are involved in pathways such as TGF-beta signaling, MAPK signaling, hyaluronan metabolism, and chondrocyte differentiation. Furthermore, CGSEA identified 45 OA-associated chemicals, including quercetin, bisphenol A, and cadmium chloride. These findings offer insight into the relationship between genes, chemicals, and their impact on OA development and progression.
Pubmed:
37749624
DOI:
10.1186/s13075-023-03164-x