mProX™ Human CHRM4 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 Reductions in CHRM4, p-AKT, and MYCN were observed in CHRM4-Knockdown PC3 cells.
Western blotting of CHRM4, p-AKT, AKT, and MYCN in PC3 cells stably transfected with the NC or CHRM4 shRNA vector, and cells were treated with 100 ng/ml NGF protein in CSS-containing medium for 1 week.
Ref: Chen, Wei-Yu, et al. "Nerve growth factor interacts with CHRM4 and promotes neuroendocrine differentiation of prostate cancer and castration resistance." Communications biology 4.1 (2021): 22.
Pubmed: 33398073
DOI: 10.1038/s42003-020-01549-1
Research Highlights
Qi A, et al. "Development of a selective and high affinity radioligand, [(3)H]VU6013720, for ." Molecular pharmacology, 2023.
The M(4) muscarinic receptors play an important role in brain regions associated with neurological disorders such as Parkinson's disease, schizophrenia, and dystonia. However, developing selective drugs that target these receptors has been a challenge, resulting in off-target effects on other muscarinic receptor subtypes. Recently, a highly selective M(4) receptor antagonist, [(3)H]VU6013720, was developed and characterized with high affinity and selectivity for the M(4) receptor compared to other subtypes. The ligand was also shown to specifically bind to M(4) receptors using rodent brain tissues and demonstrated a unique binding site that differentially affects the dissociation of the ligand from other muscarinic ligands. This new radioligand provides a valuable tool for studying M(4) receptor biology and supporting drug discovery efforts.
Pubmed:
37595966
DOI:
10.1124/molpharm.122.000643
Polzin BJ, et al. "Distinct patterns of gene expression in the medial preoptic area are related to ." BMC neuroscience, 2023.
In this study, European starlings (Sturnus vulgaris) were observed performing gregarious song, a non-sexual social behavior, in flocks. This behavior, believed to be reinforced by positive emotions, serves to increase cohesion within the group. The medial preoptic area (mPOA), a region known to play a role in gregarious song production, was examined using RNA sequencing to identify potential neurotransmitter, neuromodulator, and hormonal pathways involved in this behavior. Results showed upregulation of dopaminergic, cholinergic, and GABAergic systems, as well as genetic pathways related to glutamate signaling and social behavior in humans. The study provides insight into the mPOA's role in gregarious song and possible candidate genes for further research on social behavior in vertebrates. This data can also facilitate cross-species comparisons. In conclusion, the study sheds light on the mechanisms underlying gregarious song and its role in social interactions in European starlings.
Pubmed:
37537543
DOI:
10.1186/s12868-023-00813-4
