mProX™ Human CCKBR Stable Cell Line

Liu Y, et al. "Transcriptome profiling in rumen, reticulum, omasum, and abomasum tissues during ." Frontiers in veterinary science, 2023.
The development of the four stomachs in yaks plays a crucial role in their overall health and performance; however, the specific molecular mechanisms responsible for this relationship remain largely unknown. To address this gap in knowledge, the current study systematically examined the mRNA expression patterns of the four stomachs (rumen, reticulum, omasum, and abomasum) at five different growth time points (0 day, 20 days, 60 days, 15 months, and 3 years). Results showed that the expression patterns of differentially expressed mRNAs (DEmRNAs) were unique at birth (0 d) but became more similar at 20 d and 60 d, with further similarity observed at 15 m and adulthood. The Abomasum exhibited a markedly different expression pattern compared to the Rumen, Reticulum, and Omasum. Using the Short Time-series Expression Miner (STEM) analysis, the study found that multi-model spectra were significantly enriched over time in all four stomachs. Additionally, weighted gene co-expression network analysis (WGCNA) categorized a total of 5,486 genes into 10 different modules. The turquoise module was found to be particularly significant, containing the hub genes CCKBR, KCNQ1, FER1L6, and A4GNT, while the blue module was enriched with hub genes PAK6, TRIM29, ADGRF4, TGM1, and TMEM79. KEGG enrichment analysis revealed that both the turquoise and blue modules were involved in various metabolic pathways, including gastric acid secretion, sphingolipid metabolism, and pancreatic secretion, among others. Overall, this study aims to establish a molecular foundation for understanding the physiological functions of the rumen, reticulum, omasum, and abomasum in yaks. It further aims to elucidate the critical roles of these mRNAs in regulating the growth, development, and metabolism of yaks, and provide a theoretical basis for appropriate weaning and supplementary feeding practices for this species. The findings of this study contribute to a greater understanding of the unique physiology of yaks and have implications for their improved health and performance.
Pubmed: 37808112   DOI: 10.3389/fvets.2023.1204706

Westlund KN, et al. "Epigenetic HDAC5 Inhibitor Reverses Craniofacial Neuropathic Pain in Mice.." The journal of pain, 2023.
The authors' data suggests that HDAC5 plays a significant role as an epigenetic factor in the persistence of chronic trigeminal neuropathic pain. This is supported by their study of RNA alterations, TG neuronal excitability, and pain-related behaviors. At week 3, treatment with an HDAC5 inhibitor successfully restores RNA balance, which persists for 10 weeks. However, increased levels of response to wound healing and chronic inflammation RNAs remain. Overall, these findings highlight the potential of HDAC5 as a target for managing chronic neuropathic pain.
Pubmed: 37777035   DOI: 10.1016/j.jpain.2023.09.015