mProX™ Human CCKAR Stable Cell Line

Jing H, et al. "Identification of biomarkers associated with diagnosis of acute lung injury based ." Medicine, 2023.
Acute lung injury (ALI) is an inflammatory disease with high mortality and characterized by excessive production of inflammatory factors in lung tissue. This study aimed to identify novel diagnostic biomarkers and investigate the potential association between critical genes and immune cell infiltration in ALI. Two datasets (GSE2411 and GSE18341) were utilized to identify differentially expressed genes (DEGs) and their functions were analyzed through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. Machine learning techniques were applied to screen potential markers. Through CIBERSORT, we further investigated the infiltrating immune cells. Gene Set Enrichment Analysis was used to uncover the molecular mechanisms of critical genes. The results showed 690 DEGs, with 527 upregulated and 163 downregulated genes. Three critical genes, namely PDZK1IP1, CCKAR, and CXCL2, were identified and their correlation with various immune cells was analyzed. By using Gene Set Enrichment Analysis, we explored the specific signaling pathways involved in the critical genes and derived potential molecular mechanisms of disease progression. Additionally, transcription factors and microRNAs were predicted to be associated with the critical genes. This study provides novel insights into potential markers and the role of critical genes in ALI development.
Pubmed: 37603512   DOI: 10.1097/MD.0000000000034840

Su C, et al. "Immunotoxicity and the mechanisms of aflatoxin B1-induced growth retardation in ." Journal of hazardous materials, 2023.
Aflatoxin B1 (AFB1) is a highly toxic mycotoxin commonly found in the environment and food supply, posing serious health risks to both humans and animals. Bile acids, which are naturally synthesised from cholesterol, play a crucial role in the elimination of toxins in vertebrates. In this study, Pacific white shrimp (Litopenaeus vannamei) were used as an animal model to investigate the effects of AFB1 toxicity and the potential protective effects of bile acids. The findings from this study revealed that exposure to AFB1 resulted in inhibited growth and immune response in shrimp, along with accumulation of AFB1 and tissue damage. It was determined that AFB1 caused DNA damage, leading to activation of DNA repair mechanisms and cell cycle arrest through the ATR-cyclin B/cdc2 pathway. Moreover, AFB1 was found to directly suppress the immune response and growth of shrimp by inhibiting Toll and IMD pathways and the secretion of digestive enzymes. Interestingly, the researchers observed that dietary intake of bile acids significantly decreased AFB1 accumulation, as well as mitigated the negative effects of AFB1 on growth and immune function in shrimp. It was suggested that CCKAR, ATR, and Relish may be involved in mediating the protective effects of bile acids. This study provided novel insights into the mechanisms of AFB1 toxicity in invertebrates and highlighted the potential of bile acids as a protective agent against AFB1 toxicity.
Pubmed: 37595470   DOI: 10.1016/j.jhazmat.2023.132266