mProX™ Human CASP6 Stable Cell Line

Valcarce DG, et al. "Impact of different levels of handling on Solea senegalensis culture: effects on ." Fish physiology and biochemistry, 2023.
The first trial included fish in the fattening stage with an average weight of 226 +/- 4.96 g, while the second trial involved fish in the pre-fattening stage with an average weight of 27.20 +/- 0.44 g. The studied culture protocol, which lasted for 6 and 4 months in Trials 1 and 2 respectively, aimed at reducing handling-induced stress in the experimental tanks by minimizing routine samplings. This resulted in lower levels of cortisol in the blood plasma of the fish in both trials compared to the control group. Additionally, the proposed protocol promoted higher growth in the fish cultured under less stressful conditions in Trial 2, as evidenced by increased specific growth rates, body weight, and length. To gain further insights into the potential benefits of this protocol, the expression of key genes regulating the glucocorticoid signaling pathway and apoptosis, including glucocorticoid receptors 1 and 2 (gr1, gr2), heat shock protein 90 AA (hsp90aa), and caspase 6 (casp6), was studied in the musculoskeletal tissues from Trial 2. Consistent with the reduced cortisol levels in this trial, the expression of gr1, hsp90aa, and casp6 genes was lower in the experimental group compared to the control group. These findings have important implications for the aquaculture industry, providing valuable information for the management of stress and welfare in Solea senegalensis fish.
Pubmed: 37733196   DOI: 10.1007/s10695-023-01239-9

Li D, et al. "Liver Transcriptome Shows Differences between Acute Hypoxia-Tolerant and ." Animals : an open access journal from MDPI, 2023.
The effects of acute hypoxia, a common abiotic stress in commercial aquaculture, on fish physiology and metabolism have been extensively studied. Among the economically important fish species, the greater amberjack (Seriola dumerili) is known for its large size and rapid growth. However, due to its intolerance to hypoxia, it is susceptible to mass mortality and hinders the progress of amberjack cultivation. In an attempt to explore the molecular mechanism of acute hypoxia in greater amberjack, a comparative analysis of the liver transcriptome was performed between acute hypoxia-tolerant (HT) and -intolerant (HS) groups. Using RNA sequencing (RNA-Seq) and simulating the acute hypoxic environment, differential expression analysis revealed 829 differentially expressed genes (DEGs) between the two groups. The upregulation of genes such as slc2a5 and prkaa2, responsible for promoting sugar transport and inhibiting lipid synthesis, was observed in the HT group as compared to the HS group. On the other hand, genes that inhibit gluconeogenesis and promote lipid synthesis, such as pgp and aacs, were downregulated in the HT group. The expression of odc1, involved in intracellular antioxidant activity, was significantly downregulated in the HT group, potentially leading to decreased scavenging of reactive oxygen species (ROS). It was also found that the NF-kB pathway was activated more in the HT group compared to the HS group to resist apoptosis. Furthermore, a relative downregulation of apoptosis and autophagy-related genes, such as endog, hm13, and casp6, was observed in the HT group. This research provides important insights into the regulation mechanism employed by the liver tissue of greater amberjack to cope with acute hypoxia stress, which could help in preventing mass mortality and reducing economic losses in commercial aquaculture.
Pubmed: 37684981   DOI: 10.3390/ani13172717