mProX™ Human CACNA1I Stable Cell Line

Product Information

Target Protein

CACNA1I

Target Family

Voltage Gated Calcium Channel

Target Protein Species

Human

Host Cell Type

Neuro2A; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Autosomal Dominant Non-Syndromic Intellectual Disability; Epilepsy

Gene ID

8911

UniProt ID

Q9P0X4

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

One of the three main CaV3 voltage-gated calcium (CaV) channels that contributes to low threshold T-type currents, CACNA1I encodes the pore-forming hCaV3.3 α1 subunit. CaV3 channels feature slower closure speeds, quick inactivation, and low voltage-activation thresholds when compared to CaV channels. The CaV3.3 channels, one of the three members of the CaV3 family, have the greatest depolarized activation thresholds; they also open, inactivate, and close slowly. A small fraction of neurons, particularly GABAergic neurons in the thalamic reticular nucleus (TRN), have CaV3.3 channels, which promote oscillatory activity necessary for the production of the sleep spindle. CaV3.3 channels have a key role in mediating rebound bursts during brief membrane hyperpolarizations in TRN neurons, where they are largely inactive at resting membrane potentials. The customized CACNA1I stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Jason

The CACNA1I cell line is suitable for our high-throughput drug screening. Sep 24 2021

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chat Robert

Very easy to use for drug screening and fast turnaround too. Oct 30 2021

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FAQ

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Published Data

Fig.1 Generation and biochemical characterization of knock-in and knock-out animals.

An example of a typical representative western blot from brain tissues generated from littermates of Cacna1i+/+ (+/+) and Cacna1iRH/RH (RH/RH) demonstrating overall CaV3.3 protein levels (top panel) and CaV3.3 protein levels in crude synaptoneurosomal preparations (bottom panel).

Ref: Ghoshal, Ayan, et al. "Effects of a patient-derived de novo coding alteration of CACNA1I in mice connect a schizophrenia risk gene with sleep spindle deficits." Translational Psychiatry 10.1 (2020): 29.

Pubmed: 32066662

DOI: 10.1038/s41398-020-0685-1

Research Highlights

T-type calcium channels (Cav3.1 to Cav3.3) are involved in hormone and neurotransmitter release, sleep, and the regulation of low-threshold calcium spikes, burst firing, and rhythmic oscillations of neurons.
El Ghaleb, Yousra, et al. "CACNA1I gain-of-function mutations differentially affect channel gating and cause neurodevelopmental disorders." Brain 144.7 (2021): 2092-2106.
Pubmed: 33704440 DOI: 10.1093/brain/awab101

Recent large-scale genome-wide association studies (GWAS) have demonstrated that, in populations of European ancestry, neuronal calcium signaling plays a critical role in schizophrenia (SCZ). It is unknown, nevertheless, if genes related to the calcium signaling system are similarly linked to SCZ in the Han Chinese population.
Xie, Yijun, et al. "Further evidence for the genetic association between CACNA1I and schizophrenia." Hereditas 155 (2018): 1-5.
Pubmed: 29308060 DOI: 10.1186/s41065-017-0054-0