mProX™ Human C5AR2 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 C5AR2 was silenced in LAD2 cells
Flow cytometric analysis evaluated C5aR2 expression in control (LAD2-cntr) and C5aR2 shRNA LvP-transduced knockdown (LAD2-C5aR2kd) cells. The black histogram corresponds to cells stained with isotype control Ab, the red histogram signifies LAD2-cntr cells stained with anti-C5aR2 Ab, and the green histogram signifies LAD2-C5aR2kd cells stained with anti-C5aR2 Ab.
Ref: Pundir, Priyanka, Clayton A. MacDonald, and Marianna Kulka. "The novel receptor C5aR2 is required for C5a-mediated human mast cell adhesion, migration, and proinflammatory mediator production." The Journal of Immunology 195.6 (2015): 2774-2787.
Pubmed: 26283482
DOI: 10.4049/jimmunol.1401348
Research Highlights
Schanzenbacher J, et al. "The role of C5a receptors in autoimmunity.." Immunobiology, 2023.
The complement system is a crucial element in the body's innate immune response and plays a critical role in protecting against infections and inflammation. However, when this system is not properly regulated, it can contribute to the development of autoimmune diseases. The anaphylatoxin C5a and its receptors, C5aR1 and C5aR2, have been linked to several autoimmune disorders, highlighting their potential for targeted therapies. These receptors, found on various types of immune and non-immune cells, have distinct signaling mechanisms and contribute to both beneficial and harmful effects on the immune system. In diseases such as rheumatoid arthritis, systemic lupus erythematosus, multiple sclerosis, epidermolysis bullosa acquisita, and antiphospholipid syndrome, dysregulated C5a-mediated inflammation can worsen the autoimmune response. Targeting C5a or its receptors with small molecules or monoclonal antibodies has shown promise in clinical trials, but further research is needed to optimize patient selection, dosing, and treatment duration. In this review, the authors provide an overview of the current understanding of C5a receptor function, the implications of C5a receptors in autoimmune diseases, the underlying molecular mechanisms, and the potential for targeted therapies to modulate their activity.
Pubmed:
37598588
DOI:
10.1016/j.imbio.2023.152413
Gupta PK, et al. "Ternary model structural complex of C5a, C5aR2, and beta-arrestin1.." Journal of biomolecular structure & dynamics, 2023.
Ramaswamy H. Sarma presents a study on the role of complement component fragment 5a (C5a) in the activation of the proinflammatory modulators of the complement system. C5a is known to form binary complexes with two genomically related G protein-coupled receptors (GPCRs), C5aR1 and C5aR2. This study focuses on the structural understanding of the fully active ternary complex involving C5a, C5aR2, and beta-arrestin, a noncanonical GPCR. Through computational modeling, 500 ns molecular dynamics studies, and principal component analysis (PCA), the study provides a refined model of the C5a-C5aR2-beta-arrestin1 ternary complex embedded in a model POPC bilayer. This model will contribute to the current understanding of the interaction between beta-arrestins and the C5a-C5aR2 system.
Pubmed:
37493401
DOI:
10.1080/07391102.2023.2239927
