mProX™ Human AVPR2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1009	Magic™ Rat AVPR2 in Vitro cAMP Assay	Rat	CHO-K1	cAMP Assay

Product Information

Target Protein

AVPR2

Target Family

Vasopressin and Oxytocin Family

Target Protein Species

Human

Host Cell Type

MDCK; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research; Cardiovascular Research; Digestive and Renal Research

Related Diseases

Nephrogenic Syndrome Of Inappropriate Antidiuresis; Diabetes Insipidus; Nephrogenic; X-Linked

Gene ID

554

UniProt ID

P30518

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The G protein-coupled receptor (GPCR) superfamily includes the arginine vasopressin receptor 2 (AVPR2), which has seven transmembrane domains. The distal convoluted tubules and collecting ducts are the main locations where this molecule is expressed. Its primary role in maintaining water balance in the body and concentrating urine under physiological settings is to activate mechanisms in response to the pituitary hormone arginine pressor (AVP). The most common cause of congenital nephrogenic diabetes insipidus is mutations in this gene. Numerous malignancies, including osteosarcoma, renal cell carcinoma, breast cancer, pancreatic cancer, colorectal cancer, and small cell lung cancer, have been identified to express AVPR2. Clear cell renal carcinoma cell line proliferation is encouraged by AVPR2 activation, which is linked to tumor grade. The customized AVPR2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Astrid

The Human AVPR2 Stable Cell Line meets my requirement. Feb 17 2023

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chat Arno

I customized the mouse AVPR2 cell line for my research. Dec 02 2022

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FAQ

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Published Data

Fig.1 Stability of NDI-causing V2R mutants.

100 μM cycloheximide (CHX) was applied to MDCK cells stably expressing WT, L86P, R113Q, C192S, M272R, and W323_I324insR for 0, 4, and 8 hours before immunoblotting with anti-GFP antibody.

Ref: Prosperi, Federica, et al. "Characterization of five novel vasopressin V2 receptor mutants causing nephrogenic diabetes insipidus reveals a role of tolvaptan for M272R-V2R mutation." Scientific Reports 10.1 (2020): 16383.

Pubmed: 33009446

DOI: 10.1038/s41598-020-73089-x

Research Highlights

The diminished capacity of renal collecting duct cells to reabsorb water in response to vasopressin's antidiuretic impact is a hallmark of congenital nephrogenic diabetes insipidus (CNDI). 90% of individuals with CNDI have an X-linked hereditary disease brought on by mutations in the gene encoding the arginine vasopressin receptor 2 (AVPR2).
Joshi, Shivani, et al. "Novel and recurrent variants in AVPR2 in 19 families with X-linked congenital nephrogenic diabetes insipidus." European Journal of Pediatrics 177 (2018): 1399-1405.
Pubmed: 29594432 DOI: 10.1007/s00431-018-3132-z

These results revealed that the unique mutation in AVPR2 was a type III mutant receptor and a real disease-causing variation with mild consequences.
Guo, Shusen, et al. "Clinical and functional characterization of a novel mutation in AVPR2 causing nephrogenic diabetes insipidus in a four-generation Chinese family." Frontiers in Pediatrics 9 (2021): 790194.
Pubmed: 34956990 DOI: 10.3389/fped.2021.790194