mProX™ Human ASIC3 Stable Cell Line

Product Information

Target Protein

ASIC3

Target Family

Acid-sensing Ion Channel

Target Protein Species

Human

Host Cell Type

PANC-1; BxPC-3; SW1990; AsPC-1; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cancer Research; Inflammation Research

Related Diseases

Frozen Shoulder; Femoral Cancer

Gene ID

9311

UniProt ID

Q9UHC3

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The ASIC3 gene in humans encodes the protein known as acid-sensing ion channel 3 (ASIC3), often referred to as testis sodium channel 1 (TNaC1) or amiloride-sensitive cation channel 3 (ACCN3). Among the five paralogous genes that code for proteins that form mammalian trimeric acid-sensing ion channels (ASICs) is the ASIC3 gene. Splicing variations of the ASIC genes encode distinct proteins known as isoforms. The central and peripheral nervous systems are the primary sites of expression for these genes. Both homotrimeric (made up of three identical subunits) and heterotrimeric channels can be formed using ASICs. The customized ASIC3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Anthony

The ASIC3 cell line is easy to work with and consistently provides reliable results. May 27 2021

chat Verified Customer

chat Richard

The ASIC3 cell line was easy to use, saving me valuable time in the lab. Jan 01 2020

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 ASIC1 and ASIC3 are functionally expressed in pancreatic cancer.

RT-PCR, qRT-PCR, and western blot were used to assess the mRNA and protein expression of ASIC1 and ASIC3 in four pancreatic cancer cell lines (PANC-1, SW1990, BxPC-3, and AsPC-1) and normal pancreatic ductal cells (HPDE).

Ref: Zhu, Shuai, et al. "ASIC1 and ASIC3 contribute to acidity-induced EMT of pancreatic cancer through activating Ca2+/RhoA pathway." Cell death & disease 8.5 (2017): e2806-e2806.

Pubmed: 28518134

DOI: 10.1038/cddis.2017.189

Research Highlights

According to these findings, tiredness lowers muscle pH, which in turn triggers ASIC3 on macrophages to increase hyperalgesia in response to muscle injury.
Gregory, Nicholas S., et al. "ASIC3 is required for development of fatigue-induced hyperalgesia." Molecular neurobiology 53 (2016): 1020-1030.
Pubmed: 25577172 DOI: 10.1007/s12035-014-9055-4

Acid-sensing ion channels (ASICs) are cation-selective, trimeric, proton-activated neuronal channels that are thought to be crucial for nociception and mechanosensation.
Montalbetti, Nicolas, et al. "ASIC3 fine-tunes bladder sensory signaling." American Journal of Physiology-Renal Physiology 315.4 (2018): F870-F879.
Pubmed: 29561183 DOI: 10.1152/ajprenal.00630.2017