mProX™ Human ASIC2 Stable Cell Line

Product Information

Target Protein

ASIC2

Target Family

Acid-sensing Ion Channel

Target Protein Species

Human

Host Cell Type

SH-SY5Y; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Inflammation Research

Related Diseases

Pseudohypoaldosteronism

Gene ID

UniProt ID

Q16515

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The ASIC2 gene in humans codes for a protein called acid-sensing ion channel 2 (ASIC2), which is sometimes referred to as amiloride-sensitive cation channel 1, neuronal (ACCN1), or brain sodium channel 1 (BNaC1). Among the five paralogous genes that code for proteins that form mammalian trimeric acid-sensing ion channels (ASICs) is the ASIC2 gene.Splicing variations of the ASIC genes encode distinct proteins known as isoforms. The central and peripheral nervous systems are the primary sites of expression for these genes. Both homotrimeric (made up of three identical subunits) and heterotrimeric channels can be formed using ASICs. The customized ASIC2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Barbara

The ASIC2 cell line was easy to use, and the customer service team was responsive and helpful. Highly recommended! Mar 20 2020

chat Verified Customer

chat Michael

The technical support team was knowledgeable and provided prompt assistance whenever we had questions. I am extremely satisfied with this ASIC2 cell line and the overall experience. Oct 31 2021

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FAQ

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Published Data

Fig.1 Different subcellular localization and proton-sensitivity between ASIC2a and ASIC2b in HEK293T cells.

Sample confocal photos of HEK293T cells expressing ASIC2a or ASIC2b using an ER (mCh-Cb5) or plasma membrane (Lyn-mCh) probe. Whereas ASIC2b co-localizes with the ER marker, ASIC2a co-localizes with the plasma membrane marker.

Ref: Kweon, Hae-Jin, et al. "Acid-sensing ion channel 2a (ASIC2a) promotes surface trafficking of ASIC2b via heteromeric assembly." Scientific reports 6.1 (2016): 30684.

Pubmed: 27477936

DOI: 10.1038/srep30684

Research Highlights

By stimulating the calcineurin/NFAT1 pathway in acidosis, ASIC2 encourages the spread of CRC cells, and elevated expression of ASIC2 is associated with a poor prognosis for CRC patients.
Zhou, Zhi-hang, et al. "The acid-sensing ion channel, ASIC2, promotes invasion and metastasis of colorectal cancer under acidosis by activating the calcineurin/NFAT1 axis." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 1-12.
Pubmed: 28927426 DOI: 10.1186/s13046-017-0599-9

The role of acid-sensing ion channels (ASICs) in osteoporotic vertebral fractures (OVF) and bone cells is poorly understood. This work characterized the expression of ASICs in OVF bone cells and osteoblasts produced from adult human bone marrow mesenchymal stem cells (BM-MSCs).
Lee, Ching-Yu, et al. "High expression of acid-sensing ion channel 2 (ASIC2) in bone cells in osteoporotic vertebral fractures." BioMed Research International 2019 (2019).
Pubmed: 31531354 DOI: 10.1155/2019/4714279