mProX™ Human ASIC1 Stable Cell Line

Product Information

Target Protein

ASIC1

Target Family

Acid-sensing Ion Channel

Target Protein Species

Human

Host Cell Type

GSC; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Inflammation Research

Related Diseases

Brain Injury; Frozen Shoulder

Gene ID

UniProt ID

P78348

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The ASIC1 gene in humans encodes the protein known as acid-sensing ion channel 1 (ASIC1), often referred to as amiloride-sensitive cation channel 2, neuronal (ACCN2), or brain sodium channel 2 (BNaC2). Among the five paralogous genes that code for proteins that form mammalian trimeric acid-sensing ion channels (ASICs) is the ASIC1 gene. The central and peripheral nervous systems are the primary sites of expression for these genes. Both homotrimeric (made up of three identical subunits) and heterotrimeric channels can be formed using ASICs. The customized ASIC1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Rebecca

The ASIC1 cell line is easy to use, and the provided protocols are clear and straightforward. Its competitive pricing compared to other similar products on the market makes it an attractive option for researchers on a budget. Jun 29 2021

chat Verified Customer

chat Emily

The ASIC1 cell line is approximately lower than other brands of reagents and I got similar results. Apr 05 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 ASIC1 and ASIC3 are expressed in GSC lines.

ASIC3 is more prevalent in R8 cells than R54 cells. PCR and Western blot research revealed that ASIC1a, ASIC3, and ASIC1b are the primary ASICs expressed by R54 and R8 cells.

Ref: Tian, Yuemin, et al. "Glioblastoma cancer stem cell lines express functional acid sensing ion channels ASIC1a and ASIC3." Scientific reports 7.1 (2017): 13674.

Pubmed: 29057936

DOI: 10.1038/s41598-017-13666-9

Research Highlights

Proton-gated cation channels known as acid-sensing ion channels (ASICs) are triggered by low extracellular pH. According to recent research, ASICs may play a role in the etiology of certain malignancies, including breast and lung cancer. However, there is no information available about the impact of acidosis and ASIC activation on central nervous system malignant gliomas.
Sheng, Yulan, et al. "Acid-sensing ion channel 1 (ASIC1) mediates weak acid-induced migration of human malignant glioma cells." American Journal of Cancer Research 11.3 (2021): 997.
Pubmed: 33791169

According to this study, ASIC1 and ASIC3 are mostly expressed on the membranes of pancreatic cancer cells and are elevated in the tissues of pancreatic cancer. An acidity-induced inward current, mediated by ASIC1 and ASIC3, is necessary to raise the intracellular Ca2+ concentration ([Ca2+]i).
Zhu, Shuai, et al. "ASIC1 and ASIC3 contribute to acidity-induced EMT of pancreatic cancer through activating Ca2+/RhoA pathway." Cell death & disease 8.5 (2017): e2806-e2806.
Pubmed: 28518134 DOI: 10.1038/cddis.2017.189