mProX™ Human ADRA2A Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 Testing shRNAs for knockdown efficacy of Adra2a-IRES-gfp transgene expression in HEK293 cells.
The images display HEK293 cells after transfection, where the shAdra2a1 construct significantly diminished GFP fluorescence (in green), in contrast to the unaltered levels observed with the scramble version. Notably, dsRED expression exhibited consistent transfection efficiencies.
Ref: Zhang, Zhe, et al. "Neuronal ensembles sufficient for recovery sleep and the sedative actions of α2 adrenergic agonists." Nature Neuroscience 18.4 (2015): 553-561.
Pubmed: 25706476
DOI: 10.1038/nn.3957
Research Highlights
Ding Y, et al. "Genetic polymorphisms are associated with individual susceptibility to ." Frontiers in genetics, 2023.
The study aimed to investigate the effect of genetic polymorphisms on individual differences in the response to dexmedetomidine (DXM), commonly used as an anesthetic adjuvant or sedative. Participants (n=112) undergoing hand surgery received DXM 0.5 μg/kg followed by continuous infusion (0.4 μg/kg/h). Sedation, heart rate, and blood pressure were measured. Forty-five single nucleotide polymorphisms (SNPs) were evaluated for genotyping. Individually, ABCG2 rs2231142, CYP2D6 rs16947, WBP2NL rs5758550, KATP rs141294036, KCNMB1 rs11739136, KCNMA1 rs16934182, ABCC9 rs11046209, ADRA2A rs1800544, and ADRB2 rs1042713 were significantly associated (p<0.05) with the sedative and hemodynamic effects of DXM. Multiple linear regression analysis revealed that sex, BMI, and ADRA2A rs1800544 had a statistically significant impact on the effective dose of DXM sedation. The findings highlight the importance of these SNPs in personalized medication and improving clinical and surgical management.
Pubmed:
37693312
DOI:
10.3389/fgene.2023.1187415
Shao F, et al. "FOXO1 orchestrates the intestinal homeostasis via neuronal signaling in group 3 ." The Journal of experimental medicine, 2023.
The study investigated the role of neuro-immune regulation in maintaining intestinal homeostasis by examining the impact of a gut neuronal signal on tissue-resident lymphocytes known as intestinal group 3 innate lymphoid cells (ILC3s). The findings suggest that cyclic adenosine monophosphate (cAMP) signaling exacerbates inflammation and reduces the expression of the transcription factor forkhead box O1 (FOXO1) in ILC3s. Deficiency of FOXO1 leads to the hyperactivation of ILC3s, resulting in gut inflammation independent of T cells. FOXO1 regulates the transcription of neuropeptide receptor VIPR2 and adrenoceptor ADRA2A in ILC3s, which plays a crucial role in maintaining immune balance and intestinal homeostasis. Chronic stress increases cAMP levels and decreases FOXO1, leading to the exacerbation of intestinal inflammation. These findings demonstrate the important role of FOXO1 in regulating ILC3 activation through VIP and adrenergic signaling in maintaining intestinal homeostasis.
Pubmed:
37549024
DOI:
10.1084/jem.20230133
