mProX™ Human ADRA1D Stable Cell Line

Wang J, et al. "Functional Involvement of ADRA1D in Cutaneous Melanoma Progression and ." Cellular and molecular biology (Noisy-le-Grand, France), 2023.
Cutaneous melanoma is a highly aggressive and malignant skin cancer that poses a significant challenge in treating advanced-stage patients due to its high recurrence rate and drug resistance. A study investigated the potential of targeting the alpha-1 adrenergic receptor (ADRA1) subtypes as a treatment for melanoma in mice, but the role of the alpha-1D subtype (ADRA1D) in this cancer remains unclear. Using tissue samples and experiments on cells, researchers found that ADRA1D was expressed at low levels in cutaneous melanoma and its overexpression inhibited the growth, invasion, and proliferation of melanoma cells while also reducing angiogenesis in vivo. These findings suggest that ADRA1D may serve as a promising target in the treatment of cutaneous melanoma by regulating the HIF-1alpha/VEGF axis and inhibiting tumor growth and metastasis.
Pubmed: 37571902   DOI: 10.14715/cmb/2023.69.5.8

Zhou H, et al. "Chronic unpredictable stress induces depression/anxiety-related behaviors and ." Heliyon, 2023.
Depression and anxiety are widely recognized as the most prevalent mental health disorders, impacting individuals of all ages and disproportionately affecting females. However, despite its prevalence, the underlying pathophysiological changes associated with these disorders are not well understood. In the present study, the effects of age and stress on depression- and anxiety-related behaviors in female mice were evaluated. Specifically, saccharin preference and the open field test were utilized before and after exposure to chronic unpredictable stress in mice at 4, 14, and 25 months of age. Following behavioral testing, mRNA levels of monoamine receptors in the hippocampus were measured using real-time RT-PCR. The results indicated that chronic unpredictable stress significantly decreased saccharin preference in all age groups studied, as well as time spent in the center of the open field test in 25-month-old mice. Additionally, age and stress were found to significantly impact the expression of specific monoamine receptors, with correlations observed between saccharin preference and select receptor levels in 4-month-old mice, and between time spent in the center of the open field test and receptor levels in 4 and 14-month-old mice. These findings suggest that stress has a significant impact on both depressive and anxious behaviors, as well as the expression of monoamine receptors in the hippocampus, in a manner that is dependent on an individual's age.
Pubmed: 37539192   DOI: 10.1016/j.heliyon.2023.e18369