Magic™ Human ADRA1D Lentivirus
[CAT#: S01YF-0922-KX1586]
- Product Category:
- Membrane Protein Tools
- Subcategory:
- Membrane Protein Lentivirus
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Information
Product Overview
The lentivirus product encodes Human ADRA1D. To produce lentivirus particles, the transfer plasmid containing the Human ADRA1D, the envelope plasmid, and the packaging plasmid are co-transfected into the packaging cell line. The lentivirus particles are purified and concentrated using sucrose gradient ultracentrifugation to ensure quality. Viral titer is then determined using RT-qPCR with primers to the elements present in the viral genome. The viral particles are shipped overnight and can be used immediately for in vitro or in vivo research. The lentiviruses are packaged using 3rd generation lentiviral plasmids, and replication-incompetent.
Target Protein
ADRA1D
Target Classificationp
GPCR
Target Family
Adrenergic Family
Target Protein Species
Human
Protein Tag
Based on specific requirements (Flag, StrepII, His, etc.)
Buffer
HBSS
Serotype
HIV-1 or based on specific requirements
Titer
≧1.00E+8 TU/mL
Transfer Plasmid Backbone
pCDH or specific requirements
Size
100 uL*10 vials, 1 mL
Fluorescent Markers
EGFP, mCherry etc.
Selection Markers
Puromycin, Hygromycin, Neomycin, Blasticidin etc.
Target Research Area
CNS Research; Digestive and Renal Research
Related Diseases
Perianal Hematoma; Gastroparesis
Quality Control
Titer Determination Method
RT-qPCR
Sterility Testing
Negative
Mycoplasma Testing
Negative
Transfection Efficiency
Positive (if EGFP is included in the transfer plasmid)
Endotoxin Level
Based on specific requirements
Product Properties
Application
Stable cell line generation; Protein expression; Biochemical assays
Safety Notes
Since the HIV genes are expressed from packaging plasmids devoid of the packing signal, none of the HIV genes will be expressed in the transduced cells. The pseudotyped lentiviruses need to be used in a Biosafety Level 2 facility even if they are replication-incompetent.
Shipping
Dry ice
Storage
Store at -80°C and avoid repeated freeze-thaw cycles.
Target
Full Name
Adrenoceptor alpha 1D
Introduction
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1D-adrenergic receptor. Similar to alpha-1B-adrenergic receptor gene, this gene comprises 2 exons and a single intron that interrupts the coding region.
Alternative Names
DAR; ADRA1; ADRA1A; ADRA1R; ALPHA1; dJ779E11.2; adrenergic, alpha -1D-, receptor; adrenergic, alpha-1A-, receptor; alpha-1A adrenergic receptor; alpha-1D adrenoceptor; alpha-1D adrenoreceptor; alpha-adrenergic receptor 1a; ADRA1D; Adrenoceptor alpha 1D
Gene ID
UniProt ID
Protocols
Please visit our protocols page.
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