mProX™ Human ADGRE5 Stable Cell Line

Product Information

Target Protein

ADGRE5

Target Family

Orphan Family

Target Protein Species

Human

Host Cell Type

HT1080; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cancer Research; Orphan Receptor Research

Related Diseases

Vibratory Urticaria; Thyroid Gland Anaplastic Carcinoma

Gene ID

976

UniProt ID

P48960

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The sole member of the ADGRE family outside of immune cells is ADGRE5. In all organs, the resident or infiltrating bone marrow-derived immune cells have the greatest levels of ADGRE5, according to single cell (sc)RNAseq data. In a few tissue-specific cell types, ADGRE5 levels are low to moderate. Alveolar type 1 (AT1) and AT2 cells, respiratory ciliated cells, stomach mucus-secreting cells, smooth and skeletal muscle cells, and extravillus placental trophoblast cells are also affected. Most tumor cell lines exhibit moderate to high levels of ADGRE5, which is consistent with the parental malignancies' ADGRE5 levels. While developing the cell line and preparing it for monolayer culture, it is also possible that ADGRE5 was induced or that specific tumor cells with high ADGRE5 levels were chosen. The customized ADGRE5 stable cell line can be employed in the discovery and development of antibodies, signaling pathway researches, as well as the screening of potential therapeutic candidates.

Protocols

Please visit our protocols page.

Customer Reviews

There are currently no Customer reviews or questions for mProX™ Human ADGRE5 Stable Cell Line (S01YF-0223-KX4). Click the button above to contact us or submit your feedback about this product.

FAQ

chat Gloria (Verified Customer)

Which selection marker should I use? Feb 17 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

Creative Biolabs offers a variety of selection markers, such as puromycin (Puro), neomycin (Neo), hygromycin B (Hygro) and blasticidin (Bsd). Feb 17 2023

chat Niki (Verified Customer)

How do I select the right host cell? Sep 19 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

You'll need to take into account your research objectives. A more physiologically appropriate cell type may be the ideal choice to achieve more pertinent results if you are examining cellular metabolism or the effects of particular nutrients on cellular activities. Sep 19 2022

Published Data

Fig.1 HT1080 cells stably expressing genetically engineered CD97 protein

The expression of CD97 and EMR2 molecules in HT1080 stably transfected cells was confirmed by flow cytometry and western blotting analyses using appropriate mAbs as indicated.

Ref: Tjong, W. Y., & Lin, H. H. The RGD motif is involved in CD97/ADGRE5-promoted cell adhesion and viability of HT1080 cells. Scientific reports. 2019, 9(1), 1-12.

Pubmed: 30728423

DOI: 10.1038/s41598-018-38045-w

Research Highlights

Adhesion G protein-coupled receptors (aGPCRs) control cellular activities that are directly related to the biology of tumor cells. Individual human aGPCRs were discovered to be implicated in carcinogenesis not long after they were originally characterized.
Aust, G., et al. To Detach, Migrate, Adhere, and Metastasize: CD97/ADGRE5 in Cancer. Cells. 2022, 11(9), 1538.
Pubmed: 35563846 DOI: 10.3390/cells11091538

EMR2 demonstrated broad G protein-coupling in a yeast-based experiment in which heterologous GPCRs are coupled to chimeric G proteins, whereas CD97 coupled more selectively to Gα12, Gα13, Gα14, and Gαz chimeras.
Bhudia, N., et al. G protein-coupling of adhesion GPCRs ADGRE2/EMR2 and ADGRE5/CD97, and activation of G protein signalling by an anti-EMR2 antibody. Scientific reports. 2020, 10(1), 1-13.
Pubmed: 31969668 DOI: 10.1038/s41598-020-57989-6

In non-CNS malignancies, the surface receptor CD97/ADRGE5 is linked to the control of invasion and metastasis. Although CD97 expression is positively correlated with a bad prognosis for GBM patients, its function in this tumor is not fully understood.
Eichberg, D. G., et al. Genetic manipulation of adhesion GPCR CD97/ADGRE5 modulates invasion in patient-derived glioma stem cells. Journal of neuro-oncology. 2021, 153(3), 383-391.
Pubmed: 34028660 DOI: 10.1007/s11060-021-03778-8