mProX™ Human ACKR4 Stable Cell Line

Product Information

Target Protein

ACKR4

Target Family

Chemokine Family

Target Protein Species

Human

Host Cell Type

SUNE-1;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Immunology Research

Related Diseases

Sezary's Disease;Immune Deficiency Disease

Gene ID

Human: 51554

UniProt ID

Human: Q9NPB9

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Atypical Chemokine Receptor 4 (ACKR4) has been a focal point in various scientific studies, particularly in the realm of immunology and oncology. ACKR4, known for binding and internalizing specific chemokines, plays a crucial role in modulating the immune response. For instance, it has been discovered that ACKR4 is induced by lymph flow in endothelial cells lining lymphatic collectors, enabling them to scavenge chemokines such as CCL19 and CCL21. This scavenging mechanism is pivotal in controlling dendritic cell migration in barrier tissues, shedding light on the intricate regulation of CCL21 in vivo. Furthermore, the expression of ACKR4 has been linked to colorectal cancer progression. Studies have shown that ACKR4 is downregulated in human colorectal cancer tissues compared to normal colon tissues. Moreover, ACKR4's potential as a therapeutic target has been highlighted in the context of cardiac remodeling post-myocardial infarction. The receptor's expression also demarcates specialized vascular compartments within the spleen, supporting spleen homing of T cells. In summary, ACKR4's role in immune modulation, cancer progression, and vascular compartmentalization underscores its significance in scientific research.

Protocols

Please visit our protocols page.

Customer Reviews

There are currently no Customer reviews or questions for mProX™ Human ACKR4 Stable Cell Line (S01YF-0923-PY51). Click the button above to contact us or submit your feedback about this product.

FAQ

chat Deborah (Verified Customer)

How does ACKR4 influence cardiac remodeling after myocardial infarction? Jul 11 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

ACKR4 plays a role in alleviating cardiac remodeling post-myocardial infarction by inhibiting fibroblast IL-6 production. This suggests that ACKR4 may be a potential therapeutic target for cardiac remodeling. Jul 11 2021

chat Christopher (Verified Customer)

What is the relationship between ACKR4 and dendritic cell emigration in barrier tissues? Jul 09 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

ACKR4 regulates the migration of dendritic cells in barrier tissues by scavenging both soluble and immobilized CCL21. This mechanism helps in understanding the formation of functional chemokine gradients in vivo. Jul 09 2023

Published Data

Fig.1 Effects of ACKR4 and CCL21 on SUNE-1 cell proliferation.

Cell proliferation was assessed using the cell counting kit-8 assay, revealing significantly elevated growth rates in ACKR4 KD cells compared to the ACKR4 EV group, with ***P<0.001 and ****P<0.0001. ACKR4 denotes atypical chemokine receptor 4, while WT stands for wild-type, EV for empty vector, KD for knockdown, OE for overexpression, CCL21 for C-C motif chemokine ligand 21, and OD for optical density.

Ref: Ju, Yunhe, Chuanzheng Sun, and Xiaoli Wang. "Loss of atypical chemokine receptor 4 facilitates C‑C motif chemokine ligand 21‑mediated tumor growth and invasion in nasopharyngeal carcinoma." Experimental and therapeutic medicine 17.1 (2019): 613-620.

Pubmed: 30651842

DOI: 10.3892/etm.2018.7007

Research Highlights

Ghaffari MH, et al. "Liver transcriptome profiles of dairy cows with different serum metabotypes.." Journal of dairy science, 2023.
The study aimed to investigate differences in the hepatic transcriptome of postpartum cows with different serum metabolite profiles. The cows were divided into three metabolic clusters: high body condition score (HBCS)-predicted high BCS (HBCS-PH), HBCS-predicted normal BCS (HBCS-PN), and normal BCS-predicted normal BCS (NBCS-PN). Hepatic transcriptome analysis found a total of 13,118 genes expressed in cow liver tissue. The data also revealed distinct clusters, with the HBCS-PN group showing higher feed intake and better energy balance than the HBCS-PH group. A total of 48 differentially expressed genes were found between NBCS-PN and HBCS-PH cows, while 24 genes were found between HBCS-PN and HBCS-PH cows. These findings suggest that the liver plays a crucial role in shaping different metabolic patterns in cows.
Pubmed: 37806621 DOI: 10.3168/jds.2023-23572

Fan Y, et al. "A cell transcriptomic profile provides insights into adipocytes of porcine ." Journal of animal science and biotechnology, 2023.
The composition and developmental mechanisms of the mammary gland are crucial for the healthy growth of newborns. It is a heterogeneous organ, with its physiological function relying on the gene expression of multiple cell types. While previous studies have focused on epithelial cells, the role of neighboring adipocytes has been overlooked. In this study, the authors generated a large dataset of porcine mammary gland cells from five developmental stages. This dataset identified seven distinct cell types and revealed the distinct phenotypic and transcriptional signatures of mammary glands at different stages. The data also showed the processes involved in the differentiation, hormone sensing, and metabolic activation of epithelial cells during lactation, as well as the adaptive mechanisms of adipocytes for their own survival. The results further demonstrated the important role of immune cells in maintaining homeostasis during lactation and natural involution. The findings shed light on the molecular mechanisms involved in the dedifferentiation, proliferation, and redifferentiation of adipocytes and epithelial cells, providing a valuable resource for future research on mammary gland development and remodeling.
Pubmed: 37805503 DOI: 10.1186/s40104-023-00926-0