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  • Magic™ Human ADRB2 Lentivirus

    [CAT#: S01YF-0922-KX1591]
    Product Category:
    Membrane Protein Tools
    Subcategory:
    Membrane Protein Lentivirus

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    Lot Number

    Product Information

    Product Overview
    The lentivirus product encodes Human ADRB2. To produce lentivirus particles, the transfer plasmid containing the Human ADRB2, the envelope plasmid, and the packaging plasmid are co-transfected into the packaging cell line. The lentivirus particles are purified and concentrated using sucrose gradient ultracentrifugation to ensure quality. Viral titer is then determined using RT-qPCR with primers to the elements present in the viral genome. The viral particles are shipped overnight and can be used immediately for in vitro or in vivo research. The lentiviruses are packaged using 3rd generation lentiviral plasmids, and replication-incompetent.
    Target Protein
    ADRB2
    Target Classificationp
    GPCR
    Target Family
    Adrenergic Family
    Target Protein Species
    Human
    Protein Tag
    Based on specific requirements (Flag, StrepII, His, etc.)
    Buffer
    HBSS
    Serotype
    HIV-1 or based on specific requirements
    Titer
    ≧1.00E+8 TU/mL
    Transfer Plasmid Backbone
    pCDH or specific requirements
    Size
    100 uL*10 vials, 1 mL
    Fluorescent Markers
    EGFP, mCherry etc.
    Selection Markers
    Puromycin, Hygromycin, Neomycin, Blasticidin etc.
    Target Research Area
    CNS Research; Cardiovascular Research
    Related Diseases
    Asthma; Body Mass Index Quantitative Trait Locus

    Quality Control

    Titer Determination Method
    RT-qPCR
    Sterility Testing
    Negative
    Mycoplasma Testing
    Negative
    Transfection Efficiency
    Positive (if EGFP is included in the transfer plasmid)
    Endotoxin Level
    Based on specific requirements

    Product Properties

    Application
    Stable cell line generation; Protein expression; Biochemical assays
    Safety Notes
    Since the HIV genes are expressed from packaging plasmids devoid of the packing signal, none of the HIV genes will be expressed in the transduced cells. The pseudotyped lentiviruses need to be used in a Biosafety Level 2 facility even if they are replication-incompetent.
    Shipping
    Dry ice
    Storage
    Store at -80°C and avoid repeated freeze-thaw cycles.

    Target

    Full Name
    Adrenoceptor beta 2
    Introduction
    This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This receptor is also a transcription regulator of the alpha-synuclein gene, and together, both genes are believed to be associated with risk of Parkinson's Disease. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity, type 2 diabetes and cardiovascular disease.
    Alternative Names
    BAR; B2AR; ADRBR; ADRB2R; BETA2AR; adrenergic, beta-2-, receptor, surface; adrenoceptor beta 2 surface; beta-2 adrenoceptor; beta-2 adrenoreceptor; catecholamine receptor; ADRB2; Adrenoceptor beta 2
    Gene ID
    UniProt ID

    Protocols

    Please visit our protocols page.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR CLINICAL PROCEDURES" For licensing inquiries, please contact
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