Human FGFR2 Membrane Protein Lentivirus

Titer Determination Method

RT-qPCR

Sterility Testing

Negative

Mycoplasma Testing

Negative

Transfection Efficiency

Positive (if EGFP is included in the transfer plasmid)

Endotoxin Level

Based on specific requirements

Application

Stable cell line generation; Protein expression; Biochemical assays

Safety Notes

Since the HIV genes are expressed from packaging plasmids devoid of the packing signal, none of the HIV genes will be expressed in the transduced cells. The pseudotyped lentiviruses need to be used in a Biosafety Level 2 facility even if they are replication-incompetent.

Shipping

Dry ice

Storage

Store at -80°C and avoid repeated freeze-thaw cycles.

Full Name

Fibroblast growth factor receptor 2

Introduction

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Alternative Names

FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM; BEK fibroblast growth factor receptor; bacteria-expressed kinase; keratinocyte growth factor receptor; protein tyrosine kinase, receptor like 14; Fibroblast growth factor receptor 2

Gene ID

UniProt ID