mProX™ Human ZAP70 Stable Cell Line

Ghergus, Dana. et al. "Normal B cells express ZAP70 in chronic lymphocytic leukemia: A link between autoimmunity and lymphoproliferation?" American journal of hematology, 2023.
In chronic lymphocytic leukemia (CLL), ZAP70 has been identified as a prognostic factor due to its role in altered B-cell receptor signaling, which is significant in CLL development. Research has found a strong correlation between ZAP70 levels and the occurrence of autoimmune phenomena in CLL patients. Despite this, the root cause of CLL-associated autoimmune cytopenia, largely caused by polyclonal immunoglobulin (Ig) G produced by nonmalignant B cells, remains poorly understood. Recent findings using flow cytometry showed a substantial presence of ZAP70 expression in CD5- normal B cells from CLL patients compared to healthy subjects. This was further confirmed by detection of ZAP70 mRNA at the single-cell level, paired with polyclonal Ig heavy- and light-chain gene transcripts. These ZAP70+ normal B cells were present in various B-cell subsets, including the naïve B-cells, suggesting potential early B-cell development in CLL patients before malignant transformation. In our cohort of CLL patients, we observed a correlation between ZAP70+ normal B cells and autoimmune cytopenia, where recombinant antibodies derived from these ZAP70+ cells demonstrated autoreactivity with a high frequency, including against platelet antigens. These findings provide insights into the involvement of ZAP70 in CLL leukemogenesis and the underlying mechanisms of autoimmune complications in CLL patients.
Ghergus, Dana. et al. "Normal B cells express ZAP70 in chronic lymphocytic leukemia: A link between autoimmunity and lymphoproliferation?" American journal of hematology, 2023.
Pubmed: 37853951   DOI: 10.1002/ajh.27137

Bai, Bin. et al. "The Tyrosine Phosphatase Activity of PTPN22 Is Involved in T Cell Development via the Regulation of TCR Expression." International journal of molecular sciences, 2023.
The protein tyrosine phosphatase PTPN22 is known to inhibit T cell activation by dephosphorylating crucial proteins in the T cell receptor (TCR)-mediated signaling pathway, including lymphocyte-specific protein tyrosine kinase (Lck), Src family tyrosine kinases Fyn, and Zeta-chain-associated protein kinase-70 (ZAP70). Recently, a group of researchers successfully produced PTPN22 CS transgenic mice that displayed suppressed tyrosine phosphatase activity. Notably, these mice showed a significant reduction in thymocyte numbers, altered cytokine expression in the spleen and lymph nodes, and changes in TCRαβ-CD3 complex expression and internalization on the thymic cell surface. Furthermore, PTPN22 CS mice demonstrated altered selection of developing thymocytes and decreased levels of ZAP70, Lck, Phospholipase C gamma1 (PLCγ1), and other proteins. Overall, PTPN22 plays a crucial role in regulating TCR internalization and recycling, influencing T cell development and function in peripheral immune organs. This study provides a necessary foundation for further research on immune system construction.
Bai, Bin. et al. "The Tyrosine Phosphatase Activity of PTPN22 Is Involved in T Cell Development via the Regulation of TCR Expression." International journal of molecular sciences, 2023.
Pubmed: 37833951   DOI: 10.3390/ijms241914505