mProX™ Human VDR Stable Cell Line

Product Information

Target Protein

VDR

Target Family

Vitamin D Receptor

Target Protein Species

Human

Host Cell Type

NSCLC; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Metabolic Research

Related Diseases

Vitamin D-Dependent Rickets

Gene ID

7421

UniProt ID

P11473

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The nuclear receptor family of transcription factors includes the vitamin D receptor. The VDR gene encodes the vitamin D receptor in humans. The majority of bodily tissues express VDR, which controls gene transcription related to intestine and renal calcium and other mineral transport. Glucocorticoids reduce the expression of VDR. VDR is expressed by numerous immune cell subtypes. Calcitriol is the most powerful natural agonist, and ercalcitriol, the vitamin D2 homologue, is likewise a powerful activator. Both the secondary bile acid lithocholic acid and other forms of vitamin D bind with a lower affinity. The receptor shares a sequence with the thyroid hormone and steroid receptors and is a member of the trans-acting transcriptional regulatory factor family. The customized VDR stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Thomas

This VDR cell line is the best choice for laboratories wishing to minimise troubleshooting time and maximise data and experimental volumes. Aug 08 2020

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chat Gary

The human VDR KO cell line, often used in our laboratories. May 30 2022

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FAQ

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Published Data

Fig.1 CYP24A1 and VDR expression in NSCLC cell lines.

Expression of CYP24A1 and VDR in NSCLC cell lines in response to vitamin D analogs 1,25D3 and PRI-2191. Lung cancer cell lines were exposed to PRI-2191 and 100 nM 1,25D3 for ninety-six hours. The expression of VDR and CYP24A1 proteins was measured by western blot analysis.

Ref: Maj, Ewa, et al. "Differential response of lung cancer cell lines to vitamin D derivatives depending on EGFR, KRAS, p53 mutation status and VDR polymorphism." The Journal of steroid biochemistry and molecular biology 193 (2019): 105431.

Pubmed: 31326626

DOI: 10.1016/j.jsbmb.2019.105431

Research Highlights

This review of the literature aims to provide a current analysis of the function of the VD and VDR in the initiation, development, and prognosis of all cancer types.
Merchan, Borja Bandera, et al. "The role of vitamin D and VDR in carcinogenesis: Through epidemiology and basic sciences." The Journal of steroid biochemistry and molecular biology 167 (2017): 203-218.
Pubmed: 27913313 DOI: 10.1016/j.jsbmb.2016.11.020

The nuclear receptor vitamin D receptor (VDR) controls the biological activities of 1,25-dihydroxyvitamin D3. In the gut, VDR expression is highly expressed. Immunity, barrier function, and intestinal cell proliferation are all crucially regulated by VDR.
Bakke, Danika, and Jun Sun. "Ancient nuclear receptor VDR with new functions: microbiome and inflammation." Inflammatory bowel diseases 24.6 (2018): 1149-1154.
Pubmed: 29718408 DOI: 10.1093/ibd/izy092