mProX™ Human TRPV1 Stable Cell Line

Product Information

Target Protein

TRPV1

Target Family

TRPV

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Digestive and Renal Research; Inflammation Research; Metabolic Research

Related Diseases

Pulpitis; Bladder Disease

Gene ID

7442

UniProt ID

Q8NER1

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The TRPV1 gene in humans encodes a protein known as the transient receptor potential cation channel subfamily V member 1 (TRPV1), which is sometimes referred to as the capsaicin receptor and the vanilloid receptor 1. A component or mechanism of the mammalian somatosensory system is TRPV1. Numerous exogenous and endogenous physical and chemical stimuli can open this nonselective cation channel. TRPV1 activation results in a searing, excruciating feeling. Low pH, the endocannabinoid anandamide, N-oleyl-dopamine, and N-arachidonoyl-dopamine are some of its endogenous activators. TRPV1 receptors have been identified in numerous additional tissues, including the central nervous system, however they are mostly present in the nociceptive neurons of the peripheral nervous system. TRPV1 plays a role in both the integration of various painful stimuli and the transmission and regulation of pain. The customized TRPV1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Kevin

I have confirmed in our lab that the cells have work. Jan 27 2023

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chat James

We are very happy with the product and the turn around time, and would therefore order another reagent. May 23 2020

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FAQ

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Published Data

Fig.1 Thermal titration cryo-EM experiment and the effect of RTx on TRPV1 heat sensitivity.

A HEK-293T cell expressing rat TRPV1 was used to record a representative macroscopic current time-course (top panel) in response to a temperature ramp (10-50 °C) at a membrane potential of −60 mV. This was followed by a saturating dose of RTx (50 nM) and 20 µM ruthenium red (RR).

Ref: Kwon, Do Hoon, et al. "Vanilloid-dependent TRPV1 opening trajectory from cryoEM ensemble analysis." Nature communications 13.1 (2022): 2874.

Pubmed: 35610228

DOI: 10.1038/s41467-022-30602-2

Research Highlights

The capsaicin receptor channel, or TRPV1 channel, has been shown recently to be aberrantly expressed in a variety of tumor types in addition to immune system cells. It is linked to pain and inflammation and is triggered by heat, protons, and proinflammatory cytokines.
Bujak, Joanna Katarzyna, et al. "Inflammation, cancer and immunity-implication of TRPV1 channel." Frontiers in oncology 9 (2019): 1087.
Pubmed: 31681615 DOI: 10.3389/fonc.2019.01087

A transmembrane protein linked to pain transduction, transient receptor potential cation channel subfamily V member 1 (TRPV1) is triggered by a variety of chemical and physical stimuli.
Li, Li, et al. "The impact of TRPV1 on cancer pathogenesis and therapy: a systematic review." International journal of biological sciences 17.8 (2021): 2034.
Pubmed: 34131404 DOI: 10.7150/ijbs.59918