mProX™ Human TRPM8 Stable Cell Line

Product Information

Target Protein

TRPM8

Target Family

TRPM

Target Protein Species

Human

Host Cell Type

PC12; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Pain and Addiction Research

Related Diseases

Migraine; Lichen Sclerosus

Gene ID

79054

UniProt ID

Q7Z2W7

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Chemical ligands like menthol and icilin activate TRPM8, a temperature-sensing TRP channel. Although it is extensively expressed in prostate tissue, its exact function is yet unknown. Both trigeminal and dorsal root ganglia express TRPM8 functionally. TRPM8 is a key player in the sensory nerve system's ability to detect cold and regulate body temperature. In the endocrine pancreas, TRPM8 is not expressed. Nevertheless, TRPM8-deficient mice have elevated insulin sensitivity, which can be attributed to a compensatory mechanism that follows TRPM8−/− mice's higher insulin clearance. One compensatory mechanism that may arise from the loss of TRPM8-mediated neuronal impulses in hepatic neural innervations is the up-regulation of the insulin-degrading enzyme (IDE) in the liver. The hepatic vein is innervated by TRPM8 positive sensory afferents. TRPM8 may be a useful biomarker for pancreatic adenocarcinoma, as it has recently been linked to the disease. The customized TRPM8 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The customized TRPM8 stable cell line can be used in antibody discovery and development. Jun 16 2022

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chat Kathleen

The cell line can be used as model systems to investigate fundamental aspects of cell biology and biochemistry. Dec 11 2020

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FAQ

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Published Data

Fig.1 Co-localization of TRPM8 and NFκB in PC12 cells under cold conditions.

Co-immunoprecipitation experiments were utilized to examine the relationship between TRPM8 and NFκB in order to ascertain if NFκB mediates TRPM8 modulation of TNFα. The findings demonstrated the co-localization of TRPM8 and NFκB, as well as the strengthening of this connection in cold environments.

Ref: Wang, Xin-Pei, et al. "TRPM8 in the negative regulation of TNFα expression during cold stress." Scientific reports 7.1 (2017): 45155.

Pubmed: 28332601

DOI: 10.1038/srep45155

Research Highlights

Experimental data suggested that TRPM8 is involved in cold thermal transmission, several lethal cancers, and other diseases such as obesity, dry eye illness, migraine, and urinary tract problems.
González-Muñiz, Rosario, et al. "Recent progress in TRPM8 modulation: an update." International journal of molecular sciences 20.11 (2019): 2618.
Pubmed: 31141957 DOI: 10.3390/ijms20112618

One important cold receptor in the peripheral nervous system is the transient receptor potential ion channel (TRPM8) of the melastatin subfamily. In addition to sensory neurons, prostate epithelial cells also exhibit high levels of TRPM8 protein expression, which is controlled by androgens.
Asuthkar, Swapna, et al. "The TRPM8 protein is a testosterone receptor: I. Biochemical evidence for direct TRPM8-testosterone interactions." Journal of Biological Chemistry 290.5 (2015): 2659-2669.
Pubmed: 25480783 DOI: 10.1074/jbc.M114.610824