mProX™ Human TRPM7 Stable Cell Line

Product Information

Target Protein

TRPM7

Target Family

TRPM

Target Protein Species

Human

Host Cell Type

HNSCC; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Cardiovascular Research

Related Diseases

Amyotrophic Lateral Sclerosis-Parkinsonism/Dementia Complex; Hypomagnesemia

Gene ID

54822

UniProt ID

Q96QT4

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

TRPM7 belongs to the transient receptor potential, or TRP, ion channel subfamily that is connected to melastatin. TRP channels are typically Ca2+-permeable, frequently controlled by G-protein-coupled receptors, and associated with numerous physiological functions, such as the recognition of chemical and physical inputs. There are three subtypes of TRP channels that have been identified: the short, long, and Osm-9 subtypes. TRPM7 and its associated TRPM6 belong to the long TRP subgroup. The protein kinase domain that is linked to the ion channel's C-terminus distinguishes TRPM6 and TRPM7 from other similar proteins. Recent research has shown that TRPM6 and TRPM7 are crucial for the uptake of Mg2+ and the maintenance of homeostasis in mammals. Additionally, TRPM7 seems to be involved in a wide range of different cellular processes. The customized TRPM7 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Kathleen

The TRPM7 Stable Cell Line is great for research. Apr 25 2023

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chat Dorothy

Flow cytometry is used to verify the surface expression of the TRPM7 protein, and qPCR is used to verify the engineering results at the mRNA level. Sep 22 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Expression profiles of TRPM7 in HNSCC-derived cell lines.

TRPM7 was analyzed by immunoblotting in NHOKs and HNSCC-derived cell lines. TRPM7 mRNA expression in HNSCC-derived cell lines was measured using qRT-PCR. Target expression screening was employed for head and neck cancer cell lines.

Ref: Chen, Tsung-Ming, et al. "TRPM7 via calcineurin/NFAT pathway mediates metastasis and chemotherapeutic resistance in head and neck squamous cell carcinoma." Aging (Albany NY) 14.12 (2022): 5250.

Pubmed: 35771152

DOI: 10.18632/aging.204154

Research Highlights

TRPM7, also known as the transient receptor potential melastatin-subfamily member 7, is an ion channel that is widely expressed and possesses intrinsic kinase activity. Functional linkage between TRPM7's channel and kinase activity has been identified by molecular and electrophysiological investigations of the protein's structure and activity.
Yee, Nelson S. "Role of TRPM7 in cancer: potential as molecular biomarker and therapeutic target." Pharmaceuticals 10.2 (2017): 39.
Pubmed: 28379203 DOI: 10.3390/ph10020039

The ubiquitously produced protein known as transient receptor potential ion channel subfamily M, member 7 (TRPM7) is essential for the development of the mouse embryo.
Duan, Jingjing, et al. "Structure of the mammalian TRPM7, a magnesium channel required during embryonic development." Proceedings of the National Academy of Sciences 115.35 (2018): E8201-E8210.
Pubmed: 30108148 DOI: 10.1073/pnas.1810719115