mProX™ Human TRPC7 Stable Cell Line

Product Information

Target Protein

TRPC7

Target Family

TRPC

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Cardiovascular Research

Related Diseases

Platelet homeostasis

Gene ID

57113

UniProt ID

Q9HCX4

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The first mouse brain used to clone the TRPC7 gene. The chromosomal region 13 B2 is home to the 12 exons of the mouse TRPC7 gene. There have been eight transcripts from the mouse TRPC7 gene identified thus far. With 12 exons, the human TRPC7 gene is located on chromosome 5q31.1. Ten transcripts corresponding to human TRPC7 have been found so far. The 2,589 bp open reading frame of the TRPC7 gene in humans and mice results in an 862 amino acid protein. Human TRPC7 has a pore region and six predicted transmembrane segments, which are structurally similar to the larger TRP channel superfamily. Human TRPC7 has a pore area and six putative transmembrane segments, which are structurally similar to the wider TRP channel superfamily. Comparing human TRPC7 to mouse TRPC7, the overall identities are 98% and 75%, respectively, and 81% and 75% with human TRPC3 and TRPC6, respectively. The 12 exons that make up the rat TRPC7 gene are located on chromosome 17p14. The customized TRPC7 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat James

The Human TRPC7 Stable Cell Line meets my requirement. Jul 11 2022

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I customized the mouse TRPC7 cell line for my research. May 21 2020

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FAQ

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Published Data

Fig.1 Ultraviolet B (UVB)-induced pathology decreased in TRPC7 knockdown cells.

Senescent and nonsenescent cells in different TRPC knockdown keratinocytes were counted after a 24-hour UVB exposure, and the ratio between the two was computed. Senescence-associated β-galactosidase (SA-β-gal) staining was used to identify cell senescence.

Ref: Hsu, Wen-Li, et al. "Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B." Aging Cell 19.1 (2020): e13075.

Pubmed: 31755176

DOI: 10.1111/acel.13075

Research Highlights

TRP canonical 7 (TRPC7) is a nociceptive mechanoreceptor; mutations in the p53 gene family particularly cause TRPC7 channels to trigger the onset of UVB-induced skin aging and tumor growth.
Hsu, Wen-Li, et al. "Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B." Aging Cell 19.1 (2020): e13075.
Pubmed: 31755176 DOI: 10.1111/acel.13075

TRPC7 uses two different methods to control how automated mESC-CMs are. In addition to positively regulating the membrane clock through its impact on NCX activity, TRPC7 also positively regulates the intracellular Ca2+ clock by controlling the activities of both RyR2 and SERCA.
Liu, Xianji, et al. "TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes." Stem Cell Research & Therapy 12.1 (2021): 1-18.
Pubmed: 33941260 DOI: 10.1186/s13287-021-02308-7