mProX™ Human TRPC7 Stable Cell Line

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The first mouse brain used to clone the TRPC7 gene. The chromosomal region 13 B2 is home to the 12 exons of the mouse TRPC7 gene. There have been eight transcripts from the mouse TRPC7 gene identified thus far. With 12 exons, the human TRPC7 gene is located on chromosome 5q31.1. Ten transcripts corresponding to human TRPC7 have been found so far. The 2,589 bp open reading frame of the TRPC7 gene in humans and mice results in an 862 amino acid protein. Human TRPC7 has a pore region and six predicted transmembrane segments, which are structurally similar to the larger TRP channel superfamily. Human TRPC7 has a pore area and six putative transmembrane segments, which are structurally similar to the wider TRP channel superfamily. Comparing human TRPC7 to mouse TRPC7, the overall identities are 98% and 75%, respectively, and 81% and 75% with human TRPC3 and TRPC6, respectively. The 12 exons that make up the rat TRPC7 gene are located on chromosome 17p14. The customized TRPC7 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.