mProX™ Human TRPC5 Stable Cell Line

Product Information

Target Protein

TRPC5

Target Family

TRPC

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Pain and Addiction Research

Related Diseases

Hypertrophic Pyloric Stenosis; Pyloric Stenosis

Gene ID

7224

UniProt ID

Q9UL62

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The TRPC5 gene in humans encodes the protein known as transient receptor protein 5 (TRP-5) or short transient receptor potential channel 5 (TrpC5). A subtype of the TRPC family of transient receptor potential ion channels in mammals is called TrpC5. It is believed that the multi-pass membrane protein TrpC5 forms a non-selective calcium permeant cation channel that is activated by receptors. Either by itself or in a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4, the protein is active. Furthermore, it interacts with a variety of proteins, such as spectrin repeat-containing protein 1 (SESTD1), calmodulin, CABP1, enkurin, Na+-H+ exchange regulatory factor (NHERF), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain. The customized TRPC5 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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I purchased the Human TRPC4 Stable Cell Line hoping it would meet my project requirements. Nov 17 2022

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This product is unparalleled performance and quality. Mar 09 2022

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FAQ

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Published Data

Fig.1 Comparison of the TRPC5 structure with other TRP channel structures.

Fluorescence microscopy and biotinylation accessibility revealed that mutant proteins trafficked to the plasma membrane even while deletion of cysteine residues led to greater intracellular retention of both TRPC4 and TRPC5.

Ref: Duan, Jingjing, et al. "Cryo-EM structure of TRPC5 at 2.8-Å resolution reveals unique and conserved structural elements essential for channel function." Science advances 5.7 (2019): eaaw7935.

Pubmed: 31355338

DOI: 10.1126/sciadv.aaw7935

Research Highlights

The exacerbation of joint inflammation and hyperalgesia is the consequence of either genetic deletion or pharmacological inhibition of TRPC5. In inflammatory joint situations, activation of TRPC5 may be linked to an endogenous analgesic/anti-inflammatory mechanism.
Alawi, Khadija M., et al. "Transient receptor potential canonical 5 (TRPC5) protects against pain and vascular inflammation in arthritis and joint inflammation." Annals of the Rheumatic Diseases 76.1 (2017): 252-260.
Pubmed: 27165180 DOI: 10.1136/annrheumdis-2015-208886

The plasma membrane experiences stress as a result of mechanical forces applied to cells. When cells perceive stress, a mechanoelectric transduction cascade is triggered, which sets off compensating mechanisms. The plasma membrane's mechanosensitive ion channels are in charge of converting mechanical signals into electrical signals.
Shen, Bing, et al. "Plasma membrane mechanical stress activates TRPC5 channels." PLoS One 10.4 (2015): e0122227.
Pubmed: 25849346 DOI: 10.1371/journal.pone.0122227