mProX™ Human TRPC1 Stable Cell Line

Product Information

Target Protein

TRPC1

Target Family

TRPC

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Digestive and Renal Research; Immunology Research

Related Diseases

Pyloric Stenosis; Immunodeficiency

Gene ID

7220

UniProt ID

P48995

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

TRPC1 is a cation channel that is widely expressed and lacks selectivity. It has been suggested that TRPC1, along with Orai1 and STIM1, is involved in store-operated calcium entrance. In a range of cell types, TRPC1 plays a crucial role in agonist-, growth factor-, and protein kinase C (PKC)-induced Ca2 + entry. It also contributes to several other vital physiological processes. It has also been demonstrated that TRPC1 is involved in the migration and proliferation of cancer cells. It has been observed that glioma cell chemotaxis in response to stimuli like epidermal growth factor (EGF) requires the TRPC1 channel. According to a different study, gliomas with TRPC1 deletion had inadequate cytokinesis, which resulted in cell growth halt. It has also been shown that TRPC1 depletion inhibits the proliferation of non-small-cell lung cancer cells. In human breast cancer epithelial primary culture, TRPC1 was shown to be overexpressed and has been linked to a proliferative phenotype in the disease. The customized TRPC1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

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Published Data

Fig.1 Homer and IP3R Coimmunoprecipitate TRPC1 and Other TRPC Family Members

TRPC constructs were transfected into HEK293 cells as indicated. Every construct was tagged with HA, and the α-HA antibody (provided) was used to identify its expression.

Ref: Yuan, Joseph P., et al. "Homer binds TRPC family channels and is required for gating of TRPC1 by IP3 receptors." cell 114.6 (2003): 777-789.

Pubmed: 14505576

DOI: 10.1016/s0092-8674(03)00716-5

Research Highlights

Transient receptor potential canonical (TRPC) channels are non-selective cation channels that are calcium permeable and widely distributed across tissues. Based on amino acid sequence homology, TRPC 1/4/5 and TRPC3/6/7 are two of the seven TRPC channels that are split.
Kim, Jinsung, et al. "TRPC1 as a negative regulator for TRPC4 and TRPC5 channels." Pflügers Archiv-European Journal of Physiology 471 (2019): 1045-1053.
Pubmed: 31222490 DOI: 10.1007/s00424-019-02289-w

Two important factors that determine cell function are the voltage across the plasma membrane and the amount of free cytosolic Ca2+ present. These characteristics are known to be regulated by Ca2+-permeable non-selective cationic channels, although our knowledge of these channels is still lacking.
Rubaiy, Hussein N., et al. "Picomolar, selective, and subtype-specific small-molecule inhibition of TRPC1/4/5 channels." Journal of Biological Chemistry 292.20 (2017): 8158-8173.
Pubmed: 28325835 DOI: 10.1074/jbc.M116.773556