mProX™ Human TRPA1 Stable Cell Line

Product Information

Target Protein

TRPA1

Target Family

TRPA

Target Protein Species

Human

Host Cell Type

HaCaT; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Pain and Addiction Research

Related Diseases

Episodic Pain Syndrome

Gene ID

8989

UniProt ID

O75762

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

TRPA1 belongs to the family of transient receptor potential channels. TRPA1 is thought to act as a mechanical and chemical stress sensor since it has 14 N-terminal ankyrin repeats. A particular involvement for this protein in the peripheral nervous system's detection, integration, and initiation of pain signals is one of its specialized functions. It can be directly triggered by endogenous mediators at locations of tissue damage or inflammation, or it can be indirectly triggered as a downstream target by signaling from several different G-protein coupled receptors (GPCRs), including bradykinin. The customized TRPA1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

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FAQ

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Published Data

Fig.1 Tumor necrosis factor (TNF) induces TRPA1 expression in human HaCaT keratinocytes.

TNF was used to cultivate HaCaT keratinocytes for 48 hours. Western blotting was used to detect TRPA1 protein following protein extraction and immunoprecipitation. As a positive control, human TRPA1 plasmid-transfected HEK293 cells were employed.

Ref: Luostarinen, Samu, Mari Hämäläinen, and Eeva Moilanen. "Transient receptor potential ankyrin 1 (TRPA1)-an inflammation-induced factor in human HaCaT keratinocytes." International Journal of Molecular Sciences 22.7 (2021): 3322.

Pubmed: 33805042

DOI: 10.3390/ijms22073322

Research Highlights

A nonselective cation channel that is a member of the transient receptor potential (TRP) channel superfamily is the transient receptor potential ankyrin 1 (TRPA1) channel. It acts as an irritating sensor for many electrophilic chemicals and is mostly expressed in sensory neurons.
Chen, Jun, and David H. Hackos. "TRPA1 as a drug target-promise and challenges." Naunyn-Schmiedeberg's archives of pharmacology 388 (2015): 451-463.
Pubmed: 25640188 DOI: 10.1007/s00210-015-1088-3

The wasabi receptor, or TRPA1 ion channel, is a sensor of toxic chemicals that are either produced naturally during drug metabolism or come into contact with us externally.
Paulsen, Candice E., et al. "Structure of the TRPA1 ion channel suggests regulatory mechanisms." Nature 520.7548 (2015): 511-517.
Pubmed: 25855297 DOI: 10.1038/nature14367