mProX™ Human TNK2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1020	Magic™ Human ACK(TNK2) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

TNK2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HCC1395; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research; CNS Research

Related Diseases

Infantile-Onset Mesial Temporal Lobe Epilepsy With Severe Cognitive Regression and Ductal Carcinoma In Situ. Among its related pathways are Tyrosine Kinases / Adaptors and Oxidative damage response.

Gene ID

10188

UniProt ID

Q07912

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

TNK2 is the human gene that codes for the enzyme activated CDC42 kinase 1, or ACK1. The TNK2 gene produces ACK1, a non-receptor tyrosine kinase that interacts to many receptor tyrosine kinases, including insulin receptor (IR), EGFR, MERTK, AXL, and HER2. Additionally, in its GTP-bound state, ACK1 interacts with Cdc42Hs and suppresses both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of the protein. A distinct 47-amino acid region that is C-terminal to an SH3 domain mediates this interaction. The protein might be a part of a regulatory mechanism that maintains Cdc42Hs in its GTP-bound active form and is directly connected to a signal transduction pathway known as tyrosine phosphorylation. The customized TNK2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Peter

The TNK2 cell line is an excellent candidate for use in in vitro assays, and I highly recommend it to anyone working in this field. Jun 18 2023

chat Verified Customer

chat Lillian

The TNK2 cell line is an excellent product that has exceeded my expectations. Jan 07 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 TNK2 knockdown suppresses tumor formation.

The different tumors' expression of TNK2 or beta actin, which was found by western blotting with certain antibodies in HCC1395 xenografted tumors, is displayed.

Ref: Wu, Xinyan, et al. "The non-receptor tyrosine kinase TNK2/ACK1 is a novel therapeutic target in triple negative breast cancer." Oncotarget 8.2 (2017): 2971.

Pubmed: 27902967

DOI: 10.18632/oncotarget.13579

Research Highlights

Cancer progresses because dysregulated tyrosine kinase signaling modifies cellular homeostasis. The identification of ACK1, sometimes referred to as activated Cdc42-associated kinase 1 or TNK2, as an oncogenic kinase and a non-receptor tyrosine kinase (non-RTK), has revealed new pathways by which tyrosine kinase signaling advances the development of cancer.
Mahajan, Kiran, and Nupam P. Mahajan. "ACK1/TNK2 tyrosine kinase: molecular signaling and evolving role in cancers." Oncogene 34.32 (2015): 4162-4167.
Pubmed: 25347744 DOI: 10.1038/onc.2014.350

The disorders involving these 122 target proteins were subjected to enrichment analysis, and the findings showed that CRC had the strongest connection. Additionally, the development of colorectal cancer (CRC) was linked with 47 distinct target proteins. This adds to the evidence that the tiny chemical molecule has CRC-inhibiting properties. TNK2 was thus thought to be a viable therapeutic target for the management of metastatic colorectal cancer.
Qi, Lu, and Yanqing Ding. "TNK2 as a key drug target for the treatment of metastatic colorectal cancer." International journal of biological macromolecules 119 (2018): 48-52.
Pubmed: 30036625 DOI: 10.1016/j.ijbiomac.2018.07.124