mProX™ Human TNFRSF18 Stable Cell Line

Product Information

Target Family

Immune Checkpoint

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;MM.1S;OPM1

Target Classification

Immune Checkpoint Cell Lines

Target Research Area

Immunology Research

Related Diseases

Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-Linked; Diverticulitis

Gene ID

Human:8784

UniProt ID

Human:Q9Y5U5

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

TNFRSF18, also known as GITR, has been implicated in various biological processes and diseases. In hepatoblastoma, elevated extracellular calcium ions were found to accelerate the proliferation and migration of HepG2 cells and decrease cisplatin sensitivity. The calcium ions promoted HepG2 proliferation and migration by up-regulating the phosphorylation levels of focal adhesion kinase (FAK), protein kinase B, and p38 mitogen-activated protein kinase. In lung tumor-infiltrating Treg cells, TNFRSF18 was found to be selectively expressed in a subset of Treg cells that exhibited high suppressive activity and resistance to PD-1 blockade. In ovarian cancer, TNFRSF18 expression was significantly different between high- and low-risk groups, and the risk score was negatively associated with TNFRSF18 expression. TNFRSF18 was also found to be highly expressed in T cells of multiple primary lung cancers, suggesting it as a potential immunotherapeutic target in these cancers. Overall, TNFRSF18 has been implicated in cell proliferation, migration, immune cell infiltration, and response to therapy in various diseases.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Jordan Garcia (Verified Customer)

What is the role of TNFRSF18 in immune cell regulation? Feb 12 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

TNFRSF18, also known as GITR, plays a crucial role in the modulation of immune responses, particularly in T cell regulation and function. It has been shown to influence the activity of regulatory T cells and effector T cell responses. Feb 12 2021

chat Alex Smith (Verified Customer)

How does TNFRSF18 interact with other immune checkpoints? Aug 21 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

TNFRSF18 interacts with other immune checkpoints and plays a role in the immune response to cancer. Its expression and regulation can influence the effectiveness of immunotherapies targeting other checkpoints like PD-1 and CTLA-4. Aug 21 2021

Published Data

Fig.1 The impact of GITR on the in vitro proliferation of MM tumors

In this experiment, MM cells, including MM.1S and OPM1, underwent transfection with two distinct lentiviral vectors: PCDH empty and PCDH-GITR, both carrying GFP labeling. The assessment of cell proliferation was conducted through a GFP-based competition assay.

Ref: Liu, Yang, et al. "Novel tumor suppressor function of glucocorticoid-induced TNF receptor GITR in multiple myeloma." PloS one 8.6 (2013): e66982.

Pubmed: 23785514

DOI: 10.1371/journal.pone.0066982

Research Highlights

Xu, Haozhe. et al. "Elevated extracellular calcium ions accelerate the proliferation and migration of HepG2 cells and decrease cisplatin sensitivity." Journal of biomedical research, 2023.
Hepatoblastoma, the most common type of liver cancer in children, has been previously linked to the HepG2 cell line. These cells are known to have a propensity to form clumps while in culture. Researchers observed that the addition of calcium ions reduced the formation of these clumps and caused the HepG2 cells to disassociate. This calcium signaling pathway has been found to play a critical role in tumorigenesis. Through their study, the researchers showed that extracellular calcium ions induce changes in cell structure and promote cell migration and proliferation in HepG2 cells. Further investigation revealed that calcium ions activate several key signaling pathways, including focal adhesion kinase (FAK), protein kinase B, and p38 mitogen-activated protein kinase, which are responsible for promoting cell growth and migration. Inhibition of FAK or calcium signaling showed potential as a therapeutic strategy for HepG2 cells.
Xu, Haozhe. et al. "Elevated extracellular calcium ions accelerate the proliferation and migration of HepG2 cells and decrease cisplatin sensitivity." Journal of biomedical research, 2023.
Pubmed: 37750331 DOI: 10.7555/JBR.37.20230067

G Dykema, Arbor. et al. "Lung tumor-infiltrating Treg have divergent transcriptional profiles and function linked to checkpoint blockade response." Science immunology, 2023.
Treg cells are crucial in the preservation of equilibrium within the immune system. They possess the ability to suppress other immune cells and prevent the occurrence of autoimmune reactions. With their regulatory function, they play a significant role in maintaining immune system homeostasis. These cells also aid in the control of excessive immune responses and protect the body from being harmed by its own defenses. Overall, Treg cells play a vital role in maintaining a balanced and healthy immune system.
G Dykema, Arbor. et al. "Lung tumor-infiltrating Treg have divergent transcriptional profiles and function linked to checkpoint blockade response." Science immunology, 2023.
Pubmed: 37713507 DOI: 10.1126/sciimmunol.adg1487