mProX™ Human TLR6 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Immune Checkpoint Cell Lines
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Published Data
Fig.1 TLR6 siRNAs reduce TLR6 mRNA and protein expression in HEK293/TLR2 cells.
HEK293 constitutively expressing toll-like receptor 2 was utilized as an in vitro model system to investigate the efficacy and duration of gene knockdown achieved by RNA interference targeting the related pattern recognition receptor TLR6. On days 1 and 3 post-transfection of TLR6-specific small interfering RNAs versus non-targeting sequences, parallel analysis of cellular protein content was performed. Equivalent amounts of total protein isolated from experimental groups were fractionated electrophoretically and transferred to membranes prior to sequential incubated with primary antibodies raised against TLR6 or the housekeeping protein beta-actin, followed by horseradish peroxidase-conjugated secondary antibodies. Densitometric quantification of imaged immunoblots demonstrated robust yet transient silencing of TLR6 protein production in cells transfected with targeting versus non-targeting siRNAs. Further optimization of transfection protocols could potentiate more sustained ablation of TLR6 signaling without impacting cell viability or TLR2 expression.
Ref: Chang, Serena, Angela Dolganiuc, and Gyongyi Szabo. "Toll-like receptors 1 and 6 are involved in TLR2-mediated macrophage activation by hepatitis C virus core and NS3 proteins." Journal of Leucocyte Biology 82.3 (2007): 479-487.
Pubmed: 17595379
DOI: 10.1189/jlb.0207128
Research Highlights
Bang, Sunghee. et al. "A Cardiolipin from Muribaculum intestinale Induces Antigen-Specific Cytokine Responses." Journal of the American Chemical Society, 2023.
A systematic phenotypic screen of the mouse gut microbiome was conducted to identify metabolites with immunomodulatory properties. The screen discovered a total of 3,000 compounds with these characteristics. These results demonstrate the potential role of the gut microbiome in regulating the immune response. Further research can explore the mechanism of action and potential therapeutic applications of these metabolites. Overall, this study highlights the importance of the gut microbiome in maintaining immune homeostasis and its potential as a source of novel therapeutics.
Bang, Sunghee. et al. "A Cardiolipin from Muribaculum intestinale Induces Antigen-Specific Cytokine Responses." Journal of the American Chemical Society, 2023.
Pubmed:
37871232
DOI:
10.1021/jacs.3c09734
Jun Park, Beom. et al. "Multiple toll-like receptors (TLRs) display differential bacterial and ligand specificity in the earthworm, Eisenia andrei." Journal of invertebrate pathology, 2023.
The Toll-like receptors (TLRs) are a group of ancient and well-conserved pattern recognition receptors (PRRs) that can identify conserved pathogen-associated molecular patterns. They consist of three domains: an extracellular N-terminal domain with leucine-rich repeats (LRRs) for antigens, a type-I transmembrane domain, and an intracellular Toll/Interleukin-1 receptor (TIR) domain for downstream signaling. The authors have discovered six new full-length complementary DNA (cDNA) sequences, collectively known as Ean-TLR1/2/3/4/5/6, which contain a signal peptide, varying numbers of LRRs, and a TIR domain. Three conserved motifs, Box 1, Box 2, and Box 3, were identified within the TIR domain and are essential for downstream signaling. A phylogenetic analysis revealed two separate branches for earthworm TLRs: single cysteine cluster TLRs (sccTLRs) and multiple cysteine cluster TLRs (mccTLRs). Ean-TLRs 1/2/3/4 (sccTLRs) and Ean-TLR6 (mccTLRs) were found to be clustered with previously reported earthworm TLRs, as well as TLRs from other worm species. As PRRs, earthworm TLRs are capable of sensing a wide range of pathogens. While most earthworm TLRs were responsive to Gram-positive bacteria, only Ean-TLR3 did not show a response to any bacteria. This suggests that earthworms have the ability to distinguish between different species of Gram-positive bacteria through their TLR responses. Overall, the ligand specificity of earthworm TLRs suggests that their pathogen recognition is as diverse and specific as the mammalian TLR system.
Jun Park, Beom. et al. "Multiple toll-like receptors (TLRs) display differential bacterial and ligand specificity in the earthworm, Eisenia andrei." Journal of invertebrate pathology, 2023.
Pubmed:
37865158
DOI:
10.1016/j.jip.2023.108010
