mProX™ Human TBXA2R Stable Cell Line

Product Information

Target Protein

TBXA2R

Target Family

Prostanoid Family

Target Protein Species

Human

Host Cell Type

Hs578T, MDA-MB-231, MDA-MB-468; SUM-PT-149; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cardiovascular Research; Inflammation Research

Related Diseases

Bleeding Disorder; Phenytoin Allergy

Gene ID

6915

UniProt ID

P21731

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The G protein-coupled α isoform of the thromboxane receptor (TPα receptor), among other cell surface receptors, controls platelet function. The human TPα receptor, the first cloned human eicosanoid receptor, is a typical Class A rhodopsin-like G protein-coupled receptor (GPCR) with sequence variants in TBXA2R, which encodes the TPα receptor linked to atopic dermatitis, asthma, an autosomal dominant bleeding disorder. The TPα receptor's ligand, thromboxane A2 (TXA2), is produced by the platelet's oxidative metabolism of arachidonic acid and stimulates TP receptors in an autocrine fashion. As a result of the stimulation of Gq/11 and G12/13 heterotrimeric G proteins, RhoA and phospholipase C are activated, promoting platelet activation. The most often given antiplatelet medication, aspirin, targets the TXA2 pathway by irreversibly inhibiting cyclooxygenase enzymes (COX-1), which reduce platelet TXA2 synthesis and stimulate the TPα receptor. The customized TBXA2R stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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I purchased the mProX™ Human TBXA2R Stable Cell Line hoping it would meet my project requirements. Mar 17 2022

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The TBXA2R cell line demonstrated remarkable specificity and sensitivity, which helped us unravel complex signaling pathways. Oct 05 2022

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FAQ

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Published Data

Fig.1 TBXA2R is a basal-specific marker.

The Hs578T, MDA-MB-231, MDA-MB-468, and SUM-PT-149 TNBC cell lines, as well as the MDA-MB-231, MDA-MB-231, MDA-MB-468, and FOXC1 siRNA cell lines, were employed as a positive transfection control.

Ref: Orr, Katy, et al. "Thromboxane A2 receptor (TBXA2R) is a potent survival factor for triple negative breast cancers (TNBCs)." Oncotarget 7.34 (2016): 55458.

Pubmed: 27487152

DOI: 10.18632/oncotarget.10969

Research Highlights

The α isoform of the thromboxane receptor (TPα receptor) is one of several surface-expressed G protein-coupled receptors (GPCRs) that control platelet function. The discovery of naturally occurring uncommon GPCR variations has significantly increased with the development of genomic technologies, particularly in the TBXA2R gene, which codes for the TPα receptor.
Mundell, Stuart James, and Andrew Mumford. "TBXA2R gene variants associated with bleeding." Platelets 29.7 (2018): 739-742.
Pubmed: 30089223 DOI: 10.1080/09537104.2018.1499888

Platelet activation is tightly controlled by the thromboxane A2 receptor, lipoprotein-associated phospholipase A2, and platelet endothelial aggregation receptor-1, and aspirin absorption in the intestine is influenced by P-glycoprotein.
Peng, Ling-ling, et al. "Associations of MDR1, TBXA2R, PLA2G7, and PEAR1 genetic polymorphisms with the platelet activity in Chinese ischemic stroke patients receiving aspirin therapy." Acta Pharmacologica Sinica 37.11 (2016): 1442-1448.
Pubmed: 27641736 DOI: 10.1038/aps.2016.90