mProX™ Human STK4 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX391	Magic™ Human MST1(STK4) in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;H3347

Target Classification

Kinase Cell Lines

Target Research Area

Infectious Research

Related Diseases

Immunodeficiency 110 With Lymphoproliferation; Lung Cancer

Gene ID

Human:6789

UniProt ID

Human:Q13043

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

STK4, also known as MST1, is a serine/threonine kinase that plays a role in various diseases and conditions. In clear cell renal cell carcinoma (ccRCC), STK4 is found to be a prognostic biomarker and its expression is correlated with immune infiltrates. This suggests that STK4 may be a reliable prognostic predictor and could help identify new strategies for treating ccRCC patients. In a case report of a patient with epidermodysplasia verruciformis-like lesions, STK4 deficiency was identified as an underlying cause of the condition. The study suggests that STK4 deficiency is associated with combined immunodeficiency and skin lesions. Furthermore, STK4 is also found to be involved in the pathogenesis of other diseases such as viral skin infections and multiple myeloma. In viral skin infections, STK4 deficiency is identified in patients with unusual viral skin infections, indicating an inborn error of immunity. In multiple myeloma, STK4 negatively regulates the tumor suppressor protein TAZ and its expression is associated with poor clinical outcomes. Inhibition of STK4 leads to increased TAZ expression and sensitizes myeloma cells to frontline antimyeloma agents. Additionally, ginsenoside Rh2, a natural compound, is found to attenuate the progression of non-small cell lung cancer (NSCLC) by targeting the miR-28-5p/STK4 axis and inactivating Wnt/Œ≤-catenin signaling. This study suggests that G-Rh2 could serve as a potential therapeutic agent for NSCLC. Overall, STK4 has implications in various diseases and conditions, including cancer, immune disorders, and viral infections, highlighting its diverse applications in the field of medicine.

Protocols

Please visit our protocols page.

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FAQ

chat Cameron Jones (Verified Customer)

What roles does STK4 play in cell proliferation and migration? Sep 11 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

STK4, also known as MST1, is involved in the control of proliferation and migration of endothelial cells (EC cells). Sep 11 2022

chat Jordan Williams (Verified Customer)

How is STK4 expression related to patient survival in certain cancers? Jan 13 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

STK4 protein expression shows a trend of decrease in Type 2 endometrial cancer (EC) samples of deceased patients, indicating its potential role in cancer prognosis. Jan 13 2022

Published Data

Fig.1 Colorectal cancer cells with STK4 downregulation have a strong capacity to spread.

Migration and invasion capabilities of colon cancer cell lines were assessed via the transwell assay (as depicted in the right panels). The expression levels of STK4 were measured under conditions of STK4 knockdown or overexpression, as shown in the left panels. The data were presented as mean ± SD, based on three independent experiments. In H3347 cells, a notable increase in migrated cells was observed in the STK4-shRNA group (81 ± 18) compared to the Scr group (24 ± 12, P = 0.00006). Similarly, in the STK4-shRNA group, invaded cells were significantly higher (39 ± 13) compared to the Scr group (12 ± 4, P = 0.0062). In DLD-1 cells, the GFP-STK4 group exhibited reduced migration (18 ± 4) compared to the GFP group (34 ± 5, P = 0.037). Additionally, invaded cells in the GFP-STK4 group (33 ± 8) were significantly lower compared to the GFP group (60 ± 11, P = 0.041). The scale bar denotes 200 μm. (Scramble shRNA denoted as Scr; Migration as Mig; Invasion as Inv).

Ref: Lin, Cheng-Han, et al. "Downregulation of STK4 promotes colon cancer invasion/migration through blocking β-catenin degradation." Molecular Oncology 14.10 (2020): 2574-2588.

Pubmed: 32741119

DOI: 10.1002/1878-0261.12771

Research Highlights

Bai, Zi-Yuan. et al. "STK4 is a prognostic biomarker correlated with immune infiltrates in clear cell renal cell carcinoma." Aging, 2023.
The Mammalian STE20-like kinase 1 (MST1/STK4/KRS2) is a serine/threonine kinase that is highly conserved and a key member of the Hippo signaling pathway. Previous research has shown its significance in different types of tumors, yet its specific role in clear cell renal cell carcinoma (ccRCC) has not been thoroughly investigated. In this study, the function of STK4 in ccRCC will be systematically and comprehensively examined through various experiments and analyses.
Bai, Zi-Yuan. et al. "STK4 is a prognostic biomarker correlated with immune infiltrates in clear cell renal cell carcinoma." Aging, 2023.
Pubmed: 37870765 DOI: 10.18632/aging.205127

A Gutierrez-Marin, Paula. et al. "STK4 deficiency and epidermodysplasia verruciformis-like lesions: A case report." Pediatric dermatology, 2023.
Serine/threonine kinase 4 deficiency (STK4 or MST1, OMIM:614868) is an autosomal recessive (AR) combined immunodeficiency that can manifest with epidermodysplasia verruciformis-like lesions (EVLL) on the skin. In this study, a 17-year-old male patient, born from consanguineous parents, is presented. He has a history of recurrent respiratory infections, verruciform skin plaques, poikiloderma, chronic benign lymphoproliferation, and Sj√∂gren syndrome. He is suspected to have interstitial lymphocytic pneumonia.
A Gutierrez-Marin, Paula. et al. "STK4 deficiency and epidermodysplasia verruciformis-like lesions: A case report." Pediatric dermatology, 2023.
Pubmed: 37515487 DOI: 10.1111/pde.15400