mProX™ Human STK10 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1192	Magic™ Human LOK(STK10) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

STK10

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HeLa; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research; Cancer Research

Related Diseases

Testicular Germ Cell Tumor and Germ Cell Tumor. Among its related pathways are Signaling by Rho GTPases and RHOC GTPase cycle

Gene ID

6793

UniProt ID

O94804

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human STK10 gene encodes the enzyme serine/threonine-protein kinase 10. This gene is similar to numerous known polo-like kinases and encodes a member of the Ste20 family of serine/threonine protein kinases. The protein can phosphorylate and interact with polo-like kinase 1, and normal cell cycle progression is disrupted when a kinase-dead variant of the protein is overexpressed. By using the mitogen-activated protein kinase kinase 1 pathway, the kinase can also adversely control the production of interleukin 2 in T-cells. The customized STK10 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Kevin

Using the STK10 KO cell line from Creative Biolabs has been a game-changer for our research. Jun 07 2020

chat Verified Customer

chat Lisa

It was instrumental in our study, allowing us to specifically target STK10 proteins and investigate their role in disease. Sep 18 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Generation and validation of STK10 knockout cervical cell lines.

In order to transfect HeLa and Caski cells, the guide-RNA oligonucleotides were created, synthesized, and cloned into a pX459 vector. By sequencing the target DNA PCR products, the indel mutations in these cell lines were verified.

Ref: Zhang, Lu, et al. "Knockout of STK10 promotes the migration and invasion of cervical cancer cells." Translational Cancer Research 9.11 (2020): 7079.

Pubmed: 35117313

DOI: 10.21037/tcr-20-1601

Research Highlights

This is the first study to show that STK10 not only phosphorylates ERM proteins but also carries out a number of physiological tasks and is essential for the migration and invasion of cervical cancer cells.
Zhang, Lu, et al. "Knockout of STK10 promotes the migration and invasion of cervical cancer cells." Translational Cancer Research 9.11 (2020): 7079.
Pubmed: 35117313 DOI: 10.21037/tcr-20-1601

This is the first study to show that STK10 is critical for PCa cell migration and proliferation, which will be helpful for future research into the etiology of the illness.
Zhang, Lu, et al. "STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells." Experimental and Therapeutic Medicine 22.2 (2021): 1-8.
Pubmed: 34149897 DOI: 10.3892/etm.2021.10283