mProX™ Human SSTR1 Stable Cell Line

Product Information

Target Protein

SSTR1

Target Family

Somatostatin Family

Target Protein Species

Human

Host Cell Type

BGC823; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Intracranial Hypertension; Acromegaly

Gene ID

6751

UniProt ID

P30872

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

SSTR1 is a somatostatin receptor with a preference for somatostatin-14 over -28. This receptor and the inhibition of adenylyl cyclase are connected by pertussis toxin-sensitive G proteins. It activates pertussis toxin-insensitive G proteins, which in turn activate phosphotyrosine phosphatase and the Na+/H+ exchanger. Additionally, research has shown that the medial preoptic area, suprachiasmatic nucleus, and arcuate nucleus all have significant levels of SSTR1 and SSTR2 expression. High SSTR1 expression, however, was discovered in the ventral premammillary nucleus as well as the para- and periventricular nuclei. This means that these distributional patterns match those of the somatostatin binding sites that in vitro autoradiography was able to detect, demonstrating that a significant fraction of SSTR1 and SSTR2 receptors in the hypothalamus are linked to the perikary and dendrites of intrinsic neurons. The customized SSTR1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Quintion

The cell line worked very well. Aug 14 2022

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chat Donahue

I customized a SSTR1 knockdown cell line and it meets my requirement. Jan 12 2023

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FAQ

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Published Data

Fig.1 Knock-down of SSTR1 promoted cell-cycle progression.

Using flow cytometry, the cell-cycle percentage in AGS and BGC823 cells transfected with sh-control and sh-SSTR1, respectively, was examined. effects of SSTR1 knockdown on cell-cycle regulators p15, p21, and p27 protein expression by western blot.

Ref: Zhao, Junhong, et al. "Somatostatin receptor 1, a novel EBV-associated CpG hypermethylated gene, contributes to the pathogenesis of EBV-associated gastric cancer." British journal of cancer 108.12 (2013): 2557-2564.

Pubmed: 23722468

DOI: 10.1038/bjc.2013.263

Research Highlights

A subtype of somatostatin receptors called somatostatin receptor 1 (SSTR1) is engaged in a number of signaling processes in diverse regions of the human body. The study's findings may help in the development of stronger and more inventive SSTR1 antagonists and agonists.
Nagarajan, Santhosh Kumar, et al. "Molecular-level understanding of the somatostatin receptor 1 (SSTR1)-ligand binding: a structural biology study based on computational methods." ACS omega 5.33 (2020): 21145-21161.
Pubmed: 32875251 DOI: 10.1021/acsomega.0c02847

Studies hypothesize that SST signaling regulates the rate of NEC maturation as SCs progress along the NEC lineage, which contributes to SC quiescence and inhibition of proliferation because SST and SSTR1 are not expressed in ALDH+ cells.
Modarai, Shirin R., et al. "Somatostatin signaling via SSTR1 contributes to the quiescence of colon cancer stem cells." BMC cancer 16.1 (2016): 1-12.
Pubmed: 27927191 DOI: 10.1186/s12885-016-2969-7