mProX™ Human SPHK2 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Kinase Cell Lines
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Published Data
Fig.1 Hepatic insulin signaling is compromised by the downregulation of SphK2.
In the Huh7 hepatic cell line, a knockdown of SphK2 was achieved through the application of lentiviral-based shRNA, followed by treatment with insulin at specified concentrations for a duration of 15 minutes. Subsequent Western blot analyses were carried out.
Ref: Aji, Gulibositan, et al. "Regulation of hepatic insulin signaling and glucose homeostasis by sphingosine kinase 2." Proceedings of the National Academy of Sciences 117.39 (2020): 24434-24442.
Pubmed: 32917816
DOI: 10.1073/pnas.2007856117
Research Highlights
Chen, Yuxu. et al. "Red Rice Seed Coat Targeting SPHK2 Ameliorated Alcoholic Liver Disease via Restored Intestinal Barrier and Improved Gut Microbiota in Mice." Nutrients, 2023.
Alcoholic liver disease (ALD) is a prevalent chronic liver disease and a growing global health concern that necessitates the development of new treatments. This study aimed to evaluate the therapeutic potential of red rice extract (Monascus purpureus) in alleviating alcoholic liver injury in a mouse model. The results demonstrate the efficacy of RRME in mitigating the effects of ALD. This research highlights the potential of RRME as a novel therapeutic agent for the management of ALD.
Chen, Yuxu. et al. "Red Rice Seed Coat Targeting SPHK2 Ameliorated Alcoholic Liver Disease via Restored Intestinal Barrier and Improved Gut Microbiota in Mice." Nutrients, 2023.
Pubmed:
37836459
DOI:
10.3390/nu15194176
Dushani C U Ranasinghe, A. et al. "Altered Smooth Muscle Cell Histone Acetylome by the SPHK2/S1P Axis Promotes Pulmonary Hypertension." Circulation research, 2023.
The study delved into the enigmatic epigenetic regulation of vascular remodeling in pulmonary hypertension (PH), aiming to uncover upstream factors disrupting this equilibrium. Employing various methodologies, including RNA-seq databases and biochemical assays, it revealed that histone acetylation significantly influences transcription machinery in human pulmonary artery smooth muscle cells, particularly in PH-afflicted cells. The research also spotlighted the heightened presence of SPHK2 in iPAH lung tissue, demonstrating its pivotal role in catalyzing histone H3K9 acetylation through EMAP II. Inhibiting SPHK2 mitigated hypoxia-induced PH in mice. Furthermore, the study identified pulmonary vascular endothelial cells as key players in this epigenetic modulation process. Ultimately, these findings offer potential therapeutic avenues for mitigating PH vascular remodeling by targeting SPHK2 and histone acetylation.
Dushani C U Ranasinghe, A. et al. "Altered Smooth Muscle Cell Histone Acetylome by the SPHK2/S1P Axis Promotes Pulmonary Hypertension." Circulation research, 2023.
Pubmed:
37698017
DOI:
10.1161/CIRCRESAHA.123.322740
