mProX™ Human SLC22A8 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-0922-KX1566	Magic™ Human SLC22A8 in Vitro Drug screening assay	Human	Caco-2, MDCK, HEK293, CHO, membrane vesicles, and various transfected cells lines	Activity Assay (High throughput screening assays; Large scale single dose, duplicate profiling; IC50 profiling; Ki determination assay; Substrate determination assay )

Product Information

Target Protein

SLC22A8

Target Family

SLC Transporter

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Transporter Cell Lines

Target Research Area

Digestive and Renal Research; Metabolic Research

Related Diseases

Gout; Hyperuricemia

Gene ID

9376

UniProt ID

Q8TCC7

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human SLC22A8 gene encodes the protein known as organic anion transporter 3 (OAT3). Organic ions, some of which are pharmaceuticals and some of which are pure toxicants, are transported and excreted via OAT3. The inward sodium gradient, which propels dicarboxylate reentry into the cytosol, indirectly supports SLC22A8 (OAT3). To drive the influx of organic anions against their concentration gradient, dicarboxylates-such as alpha-ketoglutarate-are utilized as exchange substrates within cells or recycled from the external environment. The encoded protein is an integral membrane protein that is thought to be located on the renal proximal tubule cells' basolateral membrane. The customized SLC22A8 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Matthew

The SLC22A8 cell line is approximately lower than other brands of reagents and I got similar results. Apr 27 2023

chat Verified Customer

chat Kevin

The SLC22A8 cell line was easy to use, and the customer service team was responsive and helpful. Highly recommended! Dec 17 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Expression of SLC22A8 (OAT3) and it´s allelic variants in HEK293T cells line.

Elevated magnification of allelic variant R149C: HEK293T cells line expresses SLC22A8 (OAT3) and its allelic variations. Using polyethyleneimine lipofection, cells were transiently transfected with wild type protein that had been tagged with a C-terminal GFP tag.

Ref: Vávra, Jiří, et al. "Functional Characterization of Rare Variants in OAT1/SLC22A6 and OAT3/SLC22A8 Urate Transporters Identified in a Gout and Hyperuricemia Cohort." Cells 11.7 (2022): 1063.

Pubmed: 35406626

DOI: 10.3390/cells11071063

Research Highlights

The idea that OAT1 is more significant in kidney proximal tubule metabolism and OAT3 is more significant in systemic metabolism-regulating the amounts of metabolites passing through the colon, liver, and kidney-is supported by metabolomics and pathway analysis.
Bush, Kevin T., et al. "The drug transporter OAT3 (SLC22A8) and endogenous metabolite communication via the gut-liver-kidney axis." Journal of Biological Chemistry 292.38 (2017): 15789-15803.
Pubmed: 28765282 DOI: 10.1074/jbc.M117.796516

A thorough examination of the tumor microenvironment and tumor immune infiltration revealed a greater degree of general immunity in the SLC22A8 low expression group. The results of the Gene Enrichment Analysis demonstrated a correlation between low expression of SLC22A8 and immunological pathways, including humoral immune response and phagocytosis recognition. SLC22A8 expression can be employed as a predictive biomarker for ccRCC because it was found to be highly linked with both immune infiltration and survival in ccRCC.
Xu, Ke, et al. "SLC22A8: An indicator for tumor immune microenvironment and prognosis of ccRCC from a comprehensive analysis of bioinformatics." Medicine 101.37 (2022).
Pubmed: 36123895 DOI: 10.1097/MD.0000000000030270