mProX™ Human SLC22A6 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Transporter Cell Lines
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Published Data
Fig.1 Expression of SLC22A6 (OAT1) and it´s allelic variants in HEK293T cells line.
Using polyethyleneimine lipofection, cells were transiently transfected with wild type protein that had been tagged with a C-terminal GFP tag. The samples were treated with 4% paraformaldehyde, and a 400× magnification photograph was taken using a Leica DM6 microscope. In every variation, localization of the plasmatic membrane was seen. DAPI (blue) was used to label the cell nucleus.
Ref: Vávra, Jiří, et al. "Functional Characterization of Rare Variants in OAT1/SLC22A6 and OAT3/SLC22A8 Urate Transporters Identified in a Gout and Hyperuricemia Cohort." Cells 11.7 (2022): 1063.
Pubmed: 35406626
DOI: 10.3390/cells11071063
Research Highlights
This exciting work shows that renal Oat1 and Oat1/Oat3 regulatory proteins were dramatically compromised by CI and CIR, supporting dysregulation of distant sensing, signaling, and inter-organ/inter-organ communication. As a result, Oat1 may exacerbate AKI and be a therapeutic target for the early correction of this injury.
Sirijariyawat, Kungsadal, et al. "Impaired renal organic anion transport 1 (SLC22A6) and its regulation following acute myocardial infarction and reperfusion injury in rats." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1865.9 (2019): 2342-2355.
Pubmed:
31125610
DOI:
10.1016/j.bbadis.2019.05.013
According to these results, OAT1 can be utilized to enhance luciferase-expressing cell imaging and monitoring since it can transport d-luc.
Furuya, Takahito, et al. "Organic anion transporter 1 (OAT1/SLC22A6) enhances bioluminescence based on d-luciferin-luciferase reaction in living cells by facilitating the intracellular accumulation of d-luciferin." Biochemical and biophysical research communications 495.3 (2018): 2152-2157.
Pubmed:
29273507
DOI:
10.1016/j.bbrc.2017.12.102
