mProX™ Human SIGLEC8 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Immune Checkpoint Cell Lines
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Published Data
Fig.1 An elevation in cellular uptake of monomeric Aβ1-42 is facilitated by the heightened expression of Siglec-8.
The quantification of relative mean fluorescence intensities (MFI) for the uptake of monomeric Aβ between empty vector and Siglec-expressing populations was conducted. Relative values were determined for each mouse and human Siglec construct, and the data were presented as bars indicating the mean ± 95% CI, with a sample size of 6 replicates. Statistical significance was assessed using an unpaired Student's t-test, two-sided, with *P < 5e-2, **P < 1e-2, ***P < 1e-3, and ****P < 1e-4 denoting significance levels, while "ns" denoted non-significance. The mean of the 2xY->F group was represented by a dotted line.
Ref: Morshed, Nader, et al. "Phosphoproteomics identifies microglial Siglec-F inflammatory response during neurodegeneration." Molecular Systems Biology 16.12 (2020): e9819.
Pubmed: 33289969
DOI: 10.15252/msb.20209819
Research Highlights
Wang, Yucheng. et al. "Sequence variety in the CC' loop of Siglec-8/9/3 determines the recognitions to sulfated oligosaccharides." Computational and structural biotechnology journal, 2023.
Siglecs play a vital role in immune regulation by recognizing self-associated glycans and converting extracellular interactions into signals that inhibit immune cell functions. While various Siglecs have been identified to display broad specificities towards different types of sulfated oligosaccharides, recent research has revealed that Siglec-8 and Siglec-9 exhibit a high degree of specificity for sialyl 6-sulfo sugars. These findings highlight the significance of specific binding interactions between Siglecs and their target glycans in modulating immune responses.
Wang, Yucheng. et al. "Sequence variety in the CC' loop of Siglec-8/9/3 determines the recognitions to sulfated oligosaccharides." Computational and structural biotechnology journal, 2023.
Pubmed:
37675287
DOI:
10.1016/j.csbj.2023.08.014
Mesnil, Claire et al. "Lung-resident eosinophils represent a distinct regulatory eosinophil subset." The Journal of clinical investigation vol. 126,9 (2016): 3279-95.
Elevated eosinophil levels are commonly associated with infections and allergic conditions, such as asthma. However, there is also evidence suggesting that eosinophils play a role in maintaining immune balance. Recent research has characterized resident eosinophils (rEos) in the lungs of mice, revealing them as IL-5-independent Siglec-FintCD62L+CD101lo cells with a unique ring-shaped nucleus. During house dust mite-induced airway allergies, rEos remained unchanged and were accompanied by recruited inflammatory eosinophils (iEos), which were IL-5-dependent Siglec-FhiCD62L-CD101hi cells with segmented nuclei. Gene expression analysis indicated that rEos had a more regulatory profile than iEos. Mice lacking lung rEos displayed heightened Th2 cell responses to inhaled allergens, attributed to rEos' ability to inhibit the maturation of allergen-loaded DCs. Interestingly, similar phenotypic distinctions were observed between human lung rEos and iEos from eosinophilic asthmatic patients, suggesting the relevance of these findings to humans and underscoring the essential role of lung rEos in maintaining homeostasis.
Mesnil, Claire et al. "Lung-resident eosinophils represent a distinct regulatory eosinophil subset." The Journal of clinical investigation vol. 126,9 (2016): 3279-95.
Pubmed:
27548519
DOI:
10.1172/JCI85664
