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  • mProX™ Human SCTR Stable Cell Line

    [CAT#: S01YF-0923-PY81]
    Product Category:
    Membrane Protein Stable Cell Lines
    Subcategory:
    GPCR Cell Lines

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    Product Information

    Target Protein
    SCTR
    Target Family
    Glucagon Family
    Target Protein Species
    Rat
    Host Cell Type
    PC12;CHO-K1;HEK293
    Target Classification
    GPCR Cell Lines
    Target Research Area
    Cancer Research
    Related Diseases
    Gastrinoma;Esophageal Adenosquamous Carcinoma
    Gene ID
    Rat: 81779
    UniProt ID
    Rat: P23811

    Product Properties

    Biosafety Level
    Level 1
    Activity
    Yes
    Quantity
    10⁶ cells per vial
    Applications
    The Secretin Receptor (SCTR) has been identified as a potential diagnostic biomarker in colorectal cancer due to its hypermethylation. Additionally, SCTR has been associated with the regulation of various physiological processes. For instance, SCTR has been linked to the regulation of vasopressin expression and release in the hypothalamus, suggesting its role in osmoregulation. Furthermore, SCTR's involvement in the proliferation and migration of cancer cells has been explored, indicating its potential role in cancer progression. These findings highlight the importance of SCTR in various physiological and pathological processes, suggesting its potential as a therapeutic target.

    Protocols

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    FAQ

    chat Anthony (Verified Customer)

    How is SCTR hypermethylation associated with colorectal cancer? Jul 07 2021

    chat Patrick Liam (Creative Biolabs Scientific Support)

    Hypermethylation at CpG islands of the SCTR gene is a potential diagnostic biomarker in colorectal cancers and its precursor lesions. Jul 07 2021

    chat Christopher (Verified Customer)

    What role does the SCTR/AT1aR heteromer play in controlling Vp expression and release in the hypothalamus? Aug 07 2022

    chat Patrick Liam (Creative Biolabs Scientific Support)

    The SCTR-AT1aR heteromer mediates stimulatory actions of both SCT and ANGII in hypothalamic Vp expression and release as well as neuronal activities via the immediate early gene cFos. Aug 07 2022

    Published Data

    Fig.1 Suppression of Sctr leads to reduced PC12 cell proliferation while concurrently triggering apoptotic processes.

    PC12 cell lines underwent transfection with either scrambled siRNA (serving as a negative control) or siRNA oligonucleotides targeting anti-Sctr at varying concentrations of 125 ng or 250 ng. Subsequently, cellular proliferation was evaluated after a 24-hour period through the quantification of ATP levels.

    Ref: Lee, Misu, et al. "Secretin receptor promotes the proliferation of endocrine tumor cells via the PI3K/AKT pathway." Molecular endocrinology 26.8 (2012): 1394-1405.

    Pubmed: 22692904

    DOI: 10.1210/me.2012-1055

    Research Highlights

    Aiko M, et al. "Effects of Sleep Deprivation on Sleep and Sleep Electroencephalogram in ." Neuroscience research, 2023.
    Recent studies have consistently demonstrated a relationship between secretin and autism-like behavior in mice. Therefore, secretin-receptor knockout (SCTR-KO) mice are often utilized in research on autism. However, studies on the effects of secretin in humans have shown conflicting results, with some reporting improvement in autistic symptoms after secretin administration and others showing no significant effects. Additionally, it has been found that many individuals with autism spectrum disorders (ASD) also experience sleep disorders. In order to explore the relationship between secretin and sleep, researchers recorded the core body temperature and locomotor activity of SCTR-KO (-/-) and wild-type (WT) (+/+) mice before and after a 4-hour sleep deprivation period. No significant differences were observed between the SCTR-KO and control mice in the baseline condition. However, during the first dark period following sleep deprivation, an increase in non-rapid eye movement sleep was observed in the SCTR-KO group. This suggests that the absence of secretin may lead to fragmentation of sleep, making it difficult for SCTR-KO mice to maintain wakefulness. These findings are consistent with previous research showing that many individuals with ASD experience drowsiness and difficulty with focus during the day. Secretin, which functions as both an intestinal peptide and a neuropeptide in the brain, may play a role in regulating sleep as well as social cognitive behavior. Therefore, it is possible that secretin plays a role in the modulation of sleep patterns.
    Pubmed: 37774845   DOI: 10.1016/j.neures.2023.09.008

    Berg P, et al. "Loss of the Secretin Receptor Impairs Renal Bicarbonate Excretion and Aggravates ." Journal of the American Society of Nephrology : JASN, 2023.
    Evidence suggests that during acute base excess, the renal collecting duct beta -intercalated cells ( beta -ICs) are activated to increase urine base excretion. This process depends heavily on the presence of pendrin and cystic fibrosis transmembrane regulator (CFTR) in the apical membrane of the beta -ICs. However, the specific signal that triggers this activation remained unknown. Recent studies have shown that plasma secretin levels rise during acute alkalosis, and that the secretin receptor (SCTR) is functionally expressed in the beta -ICs. In fact, in mice with global knockout for the SCTR, the ability to acutely increase renal base excretion is lost. This results in a compensatory decrease in ventilation to cope with the acid-base challenge. These findings suggest that secretin plays a crucial role as a systemic bicarbonate-regulating hormone, likely being released from the small intestine during alkalosis. Further investigation revealed that the SCTR system is essential for renal base excretion during acute metabolic alkalosis. These findings were established through various experiments, including bladder catheterization, metabolic cage studies, blood gas analysis, barometric respirometry, and perfusion of isolated cortical collecting ducts. The study also involved the use of SCTR wild-type and knockout (KO) mice, as well as isolated rat small intestines to study secretin release. Results showed that in wild-type mice, secretin was able to acutely increase urine pH and pendrin function in isolated perfused cortical collecting ducts, which were absent in KO mice. The KO mice also failed to sufficiently increase renal base excretion during acute base loading and developed prolonged metabolic alkalosis when exposed to acute oral or intraperitoneal base loading. Furthermore, the KO mice exhibited transient but significant hypoventilation after base loading. This suggests that the loss of SCTR impairs the appropriate increase of renal base excretion during acute base loading, and that SCTR is necessary for the acute correction of metabolic alkalosis. Additionally, results from perfused rats showed that increased blood alkalinity in the upper small intestine led to higher secretin release. In conclusion, the findings suggest that secretin plays a critical role in maintaining bicarbonate homeostasis and that its release from the small intestine is triggered by blood alkalinity.
    Pubmed: 37344929   DOI: 10.1681/ASN.0000000000000173

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR CLINICAL PROCEDURES" For licensing inquiries, please contact
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